The quantitative PCR was performed with SYBR Green Master Mix (Q711-02, Vazyme, Nanjing, China) in a Real-Time PCR System (QuantStudioTM 3, Thermo Fisher Scientific, CA, USA). Gene levels were normalized to β-actin, and the relative gene expression was analyzed using the ΔΔ-Ct algorit...
Structure determination of TAK-925-bound OX2R coupled to Gqand Gi1 To capture active structures of OX2R bound to TAK-925, we purified complexes of the receptor together with heterotrimeric G proteins. Using recombinant protein from Sf9 insect cells, we elucidated the cryo-EM structure of OX2...
Statistical analysis was performed using GraphPad Prism 6.04 (GraphPad Software, Inc., San Diego, CA) or EXSUS statistical software package (CAC Croit Corporation, Tokyo, Japan). Student'st-tests (for homogeneous data) or Aspin–Welch's tests (for non-homogeneous data) were used to assess the...
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