目的 探讨Toll样受体-4(Toll like receptor-4,TLR-4)抑制剂TAK-242对大鼠重度牙周炎骨质吸收的影响,为重度牙周炎寻找辅助治疗手段提供实验基础。方法 18只3周龄雄性Wistar大鼠随机分为3组(n=6),其中1组为正常对照
Tak242, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
2、将Raji细胞分为对照组(培养基培养)24h和(10、60)μmol/LTAK-242 处理24h组,用Transwell TM 法检测细胞侵袭情况。 3、反转录PCR法检测对照组(培养基培养)24h和(10、60)μmol/LTAK-242 处理24h组TLR4、NF-κBp65的mRNA水平。 4、WesternBlot法检测对照组(培养基培养)24h和(10、60)μmol/LTAK-242 处...
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For muscle injury experiments, 100 ml of 1.2% BaCl2 (Sigma Chemical Co.) dissolved in saline was injected into the TA muscle of mice. For one experiment, mice were given intraperitoneal injection of EdU (100 mg per mouse) at day 3 or day 5 after intramuscular injection of 1.2% BaCl2. ...
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The general protein translation inhibitor CHX (Sigma-Aldrich) was used as 0.25 mg/ml (Figure 1c) and 5 mg/ml (Figure 3a). Nec-1 (Calbiochem, Merck KGaA, Darmstadt, Germany) was used at 10 mM. The TAK1 kinase inhibitor, NP-009245 (AnalytiCon Discovery GmbH, Potsdam, Germany), a ...
1.1.2 主要材料和仪器 P.gingivalis ATCC33277 (口腔疾病研究国家重点实验室提供);甲基绿试剂盒(Sigma,美国);抗酒石酸酸性磷酸酶试剂盒 (Sigma,美国);TAK⁃242(MCE,美国);氯化血红素;维生素 K;酵母提取物;TSB 培养基(成都达硕 实验有限公司,中国);5⁃0 无菌医用丝线;Quantum 口腔疾病防治 2022 年 3 月...
Analysis of variance (ANOVA) was performed with SigmaStat version 3.1 for Windows (Systat Software, Point Richmond, CA, USA). Tumor volumes were compared over time between untreated and treated groups by pairwise two-way ANOVA followed by the Tukey’s honestly significant difference test. Abbrevia...
Sytox Red Dead Cell Stain, DHR123 (Molecular Probes–Invitrogen, Eugene, OR, USA), and propidium iodide (Sigma Aldrich, Steinheim, Germany) were used at, respectively, 5 nM, 1 mM, and 3 mM. The following antibodies were used for L929 cells: antibody against cIAP1 and cIAP2 (RIAP1 ...