Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 背景介绍 DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is...
10×Biotin Ligase Buffer B:100mM ATP, 100mM MgOAc, 500μM D-Biotin D-Biotin:500μM D-Biotin 产品用途: 生物素受体蛋白的生物素化,特别是AviTag融合蛋白的生物素化,可以位点特异性的Biotin修饰目标蛋白,用于亲和纯化,基于biotin-avdin/streptavidin的检测如BIAcore传感片SA的亲和力检测等。由于修饰位点在标签...
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Storage Conditions Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 背景介绍 Protein tags are protein or peptide sequences located either on the C- or N- terminal of the...
ADDITIONAL MATERIALS REQUIRED T4 DNA ligase Taq DNA polymerase Taq DNA polymerase buffer TE buffer CIAP Falcon 2059 polypropylene tubes (15-ml) Water baths (37°C and 42°C) XL1-Blue Genotype: recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)]. ...
5)弃下层管中液体,最高转速离心1min,甩掉管中的水珠; 6)加入25μL 50℃预热的dd H2O洗脱产物,放置片刻,最高转速离心1min,回收PCR产物。采用10μL体系完成连接反应,其中取8.5μL纯化产物作为模板,加入1.0μL T4DNA ligase Buffer以及0.5μL T4 DNA ligase,于连接仪中16℃过夜(约20h)或22℃连接2h。©...
Full NameMyc tag Gene SymbolMyc tag Gene ID (NCBI)99 ConjugateUnconjugated FormLiquid Purification MethodN/A Storage BufferPBS with 0.02% sodium azide and 50% glycerol , pH 7.3 Storage ConditionsStore at -20°C. Stable for one year after shipment.Aliquoting is unnecessary for -20oC storage.20...
Genomic DNA was prepared by lysing cell pellets in lysis buffer (10 mM Tris-HCl pH 8.0, 5 mM EDTA, 100 mM NaCl, 1% SDS, 0.5 mg ml−1 proteinase K) overnight at 55 °C. After cell lysis, RNA A treatment (Qiagen, 100 mg ml−1, 1:1,000 dilution) was...
441 thermofisher.com/contactus V5 Tag Monoclonal Antibody (SV5-Pk1) Catalog Number R960-25 Details Size Host/Isotope Class Type Clone Immunogen Conjugate Form Purification Storage buffer Contains Storage Conditions 50 µL Mouse / IgG2a Monoclonal Antibody SV5-Pk1 V5 synthetic peptide: Gly-Lys-...
1.9 ml membrane isolation buffer was used to wash the membrane pellet, followed by a second centrifugation (36000 g, 40 min, 4°C). The membrane pellet was then dissolved in 12.5 ml membrane isolation buffer containing 0.1% (v/v) sarcosyl (N-laurylsarcosine sodium salt solution...
Aqueous buffer solution Formulation 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 0.4% Tween- 20, and 20% glycerol MW 116 kDa Amino Acids 2-794 Genbank # NM_015323 UniProt # O94874 Tag(s) N-terminal GST-tag Background UFL1 (E3 UFM1-protein ligase 1), also known as RCAD...