Methods GUCY2C-TAC receptors were humanized using rational mutational changes to the nanobody framework amino acids. The resulting constructs were functionally characterized using in vitro and in vivo models. In vitro assays included T cell proliferation, repeat killing assay, and cytotoxicity via ...
In cancer, however, GUCY2C is frequently overexpressed in primary and metastatic colorectal carcinomas and, thus, a preferred antigen for the specific targeting of tumor cells via TAC T cells. Methods GUCY2C-TAC receptor functionality was characterized using a variety of in vitro and in vivo ...