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It is linked to the sample surface temperature through Planck's law of blackbody radiation (Equation 1): Lλ(T ) = ελ (T ) ⋅ Lλb(T ) = ελ (T ) ⋅ c1L n2 ⋅ λ5 ⋅ expn ⋅ c2 λ⋅ T − 1...
in the presence of air, ATP, Mg2+ and DTT. Consequently, a series of experiments was performed to elucidate the nature of the activating species. More spe- cifically, samples of NrdB∆169apo were prepared under conditions analogous to those used in the enzymatic assays, albeit at ...