3. ^ "TA cloning protocol". Durham, New Hampshire, USA: Hubbart Center for Genomic Studies. http://hcgs.unh.edu/protocol/basic/Cltaclone.html. Retrieved 2009-10-17. 4. ^ "TA Cloning Kit Manual". Invitrogen. 7 April 2004. pp. 31. http://stanxterm.aecom.yu.edu/wiki/data/Product_m...
Updated Protocol for TA Cloning & Zero Blunt Kits We're excited to announce that we've made our TA Cloning® and Zero Blunt® kits even better with the introduction of our ExpressLink™ T4 DNA Ligase for faster ligations at room temperature. After downloading the appropriate protocol ...
Protocol: TA cloning ligation reaction Two or three ligation reactions for each PCR product should be assembled in order to maximize cloning efficiency: remember that the precise ratio ofvector to insert influences the ligation efficiency. Note that multiple ligations are suggested only for first-time...
TOPO™ TA Cloning™ 试剂盒设计用于仅在 5 分钟内即可从 PCR 反应中直接克隆 PCR 产物 (1)。它们使用含共价结合拓扑异构酶 I 的 pCR™-TOPO™ 载体进行快速克隆和重组。pCR™-TOPO™ 载体包括:•3´-T 突出端,用于直接连接 Taq 扩增PCR 产物• 可选择 T7 (pCR™2.1-TOPO™) 或 T7...
T-Vector cloning: The easy way to clone PCR products. Use the pGEM®-T vectors for general PCR cloning and the pTargeT™ Vector when you need to express the insert in mammalian cells.
In this study, I developed a series of three types of PCR cloning vectors to meet the need for a vector suitable for both TA and blunt-end cloning. Further, I demonstrate here the utility of this vector series using a direct PCR cloning protocol. This protocol inserts PCR products into no...
The cloning of a PCR-generated product, whether it has been amplified from cDNA or genomic DNA, is an essential step for many biologists. Traditionally, cloning was achieved by the “restriction enzyme cutback and ligation” method, where primers were designed with restriction sites to allow for...
kits include the pCR™II-TOPO® TA vector with dual T7 and SP6 promoters. By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable cloning method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinant...
By eliminating time-consuming and tedious restriction site cloning, TOPO® cloning is the most reliable cloning method, featuring a 3-step protocol and 5-minute cloning reaction, and yielding up to 95% recombinants.Convenient features of this TOPO® TA Cloning® Dual Promoter kit include:...
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