T7 Primer Sequence:5´- TAATACGACTCACTATAGGG- 3´ 仅供科研使用。不可用于诊断程序。 规格 促进剂T7 产品类型引物 引物长度20-mer 引物序列5´d[TAATACGACTCACTATAGGG]3´ 纯化方法凝胶纯化 运输条件室温 引物T7 数量2 μg 适用于(应用)PCR 扩增 ...
所以T7聚合酶的工作方式就是:识别正义链的T7启动子序列,然后以反义链为模板,按照5'-3'的方向进行转录,转录生成的序列应当是与正义链完全一致的序列。 FAQ: Does the transcription reaction with T7 RNA Polymerase require a primer? No, T7 RNA Polymerase recognizes its specific promoter sequence and start...
T7 Promoter Primer features include: • Desalted and purified by gel filtration • Assayed for function by PCR amplification • Provided in 2 μg quantity Applications • Sanger sequencing • PCR amplification T7 Primer Sequence:5´- TAATACGACTCACTATAGGG- 3´ ...
产品名称:T7 Terminator Reverse Primer (19-mer) .0订购此产品 供应商:NEB 规格:0.5 A260 U 目录价:￥:1209 库存状态: CAS编号: 应用范围: 种属来源: 相关信息: 保存条件: 说明书地址:点击查看详细 打印此页关闭此页 上一个:Fat I[V0156L/250 U] ...
• Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence. Catalog and Promoter sequences: • SO118—T7 promoter sequencing primer, 20-mer 5'-d (TAATACGACTCACTATAGGG)-3' Note
Promoter sequences5'-d (TAATACGACTCACTATAGGG)-3' Related products T3 promoter Sequencing Primer, 17-mer SP6 promoter Sequencing Primer, 24-mer T3 promoter Sequencing Primer, 24-mer SP6 promoter Sequencing Primer, 18-mer Notes • Primers cannot be used for certain plasmids that contain trunca...
• Functionally tested by dideoxy sequencing of a vector containing an appropriate RNA polymerase promoter sequence. Catalog and Promoter sequences: • SO118—T7 promoter sequencing primer, 20-mer 5'-d (TAATACGACTCACTATAGGG)-3' Note
dideoxy sequencing is the Escherichia coli DNA-polymerase I large fragment ("Klenow"). Another polymerase used is AMV reverse transcriptase. SUMMARY OF THE INVENTION In one aspect the invention features a method for determining the nucleotide base sequence of a DNA molecule, comprising annealing the...
RT extends the primer into a complementaryDNA sequence. The RNA strand of the formed RNA/DNA duplex is then degraded byRNase Hthus allowing the reverse primer P2 to hybridize the ssDNA and RT to extend P2 primer thus producing a dsDNA that contains the T7 promoter sequence. After the initia...
Functional and sequence analysis of resulting artificial genes revealed that number of deletions, insertions and substitutions was strongly reduced when T7 endonuclease I was used for mutation removal. This method diminished mutation frequency by eightfold relative to gene synthesis not incorporating an ...