T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity. T7 RNA聚合酶催化T7启动子介导的5’→3’RNA合成。它是克隆自T...
A duel-plasmid gene expression system of T7 bacteriophage components was developed; one containing the T7 phage RNA polymerase gene under the control of eukaryotic promoter CMV (pCMV-T7pol) and the other (pT7IRES) containing the T7 promoter and T7 terminator as well as EMCV IRES. To test...
The multiple cloning site of pET23a (or pET23d) vector. Restriction enzyme sites present in the multiple cloning regions are indicated. Primers for the T7 promoter and T7 terminator region of pET23 are represented by arrows. 阅读了该文档的用户还阅读了这些文档 2 p. Intercom Power Supply ...
Reliable transcription.T7 RNA Polymerase catalyzes the 5′→3′ RNA synthesis from the T7 promoter. It is a DNA-dependent RNA polymerase cloned from the T7 bacteriophage, which recognizes the T7 promoter and terminator sequences with high specificity.Specifications...
Additionally, the vectors contain the T1T2 transcriptional terminator, the pMB1 (derivative of pBR322) origin of replication, the f1 origin and the lacI gene for repression of the T7 promoter. pT7-FLAG® and pT7-MAT™ vectors offer the very strong T7/lac promoter. These expression ...
pT7andtheT7terminator(Figs.5.15.15.15.2;alsoseeCriticalParameters).This recombinantsmidisthenintroducedvialiposome-mediatedtransfection(UNIT5.10) intocellsalreadyinfectedwithvacciniavTF7-3(arecombinantexpressing theT7RNApolymerasegene).Afterharvesting,thecellsareyzedforexpressionof ...
① 复制起始点ori ② Bom ③ Rop ④ lacI promoter +lacI ⑤ T7 promoter ⑥ Lac operator ⑦ RBS ⑧ 6×His Tag ⑨ Thrombin site ⑩ S-Tag ⑪ Enterkinase site ⑫ MCS ⑬ T7 terminator ⑭ F1 ori ⑮ KanR pet-30a(+)多克隆位点 ...
(Internal Ribosomal Entry Site) elements, as well as upstream of polyA sequence and T7 terminator (Fig.1A). Since mRNA produced in the cytoplasm lacks a 5′-cap that stabilizes transcripts and recruits the translational machinery21, the sequences of viral IRES elements, as an alternative ...
promoter. In the presence of arabinose the binding of one member of the dimer changes, such that the loop is released, and it binds at another site that stimulates expression. This system results in very low background expression. Thus, the repressor gene associated with thearaBADpromoter ...
Analysis of the terminator region after the deoCABD opcron of Escherichia coli K-12 using a new class of single copy number operon-fusion vectors The new vectors are miniderivatives of plasmid Rl containing the parB stability locus of this plasmid and the lac genes as a selectable marker. Sin...