While cardiovascular disease (CVD) is one of the major co-morbidities in patients with rheumatoid arthritis (RA), the mechanism(s) that contribute to CVD in patients with RA remain to be fully elucidated. Herein, we observe that plasma concentrations of
CO2 was trapped in 250-μl microcentrifuge tubes containing 1 m NaOH by incubating the tubes at room temperature for 1 h with shaking. The acidified media were incubated overnight with 15% BSA and then centrifuged at 14,000 × g for 20 min. Aliquots of the supernatant were counted for ...
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After incubation for 30 min in a rotor at 4 °C, the cell suspension was centrifuged for 15 min at 14,000× g at 4 °C, and the supernatant was transferred into new tubes. Half of the supernatant was then incubated overnight with 2 μg of either anti-goat IgG or anti-DDIT4 ...