Ligation Protocol with T4 DNA Ligase (M0202) Golden Gate (24 Fragment) Assembly Protocol Golden Gate Assembly Protocol using PaqCI (NEB #R0745) and T4 DNA Ligase (NEB #M0202) 使用指南 Activity of DNA Modifying Enzymes in rCutSmart™ Buffer Tips for Maximizing Ligation Efficiencies 应用实例 ...
Rapid ligation protocol T4 DNA Ligase High coverage gene expression profiling on the Applied Biosystems® 3500xL Genetic Analyzer--a sensitive method for detecting gene transcripts Product Information T4 DNA Ligase 5U/ul 常见问题解答 (FAQ)
Rapid ligation protocol T4 DNA Ligase High coverage gene expression profiling on the Applied Biosystems® 3500xL Genetic Analyzer--a sensitive method for detecting gene transcripts Product Information T4 DNA Ligase 5U/ul 常见问题解答 (FAQ)
T4 DNA Ligase中文使用说明 Thermo 下载积分: 100 内容提示: PRODUCT INFORMATION T4 DNA Ligase, 5 u/µl #EL0011 Lot Store at -20°C www.thermoscientific.com/fermentas 1000 u Expiry Date Description T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5??phosphate ...
T4 DNA LigaseM0202MVIAL-201 x 0.05 ml2,000,000 units/ml T4 DNA Ligase Reaction BufferB0202SVIAL-201 x 1 ml10 X 特性和用法 单位定义 1 单位是指在 20 µl 反应体系,1X T4 DNA 连接酶反应缓冲液中,16℃ 条件下 30 分钟内能使 50% 的经 HindIII 消化的 λ DNA 片段 [DNA 5´ 末端浓...
Ligation Protocol with T4 DNA Ligase (M0202) Golden Gate (24 Fragment) Assembly Protocol 使用指南 Activity of DNA Modifying Enzymes in rCutSmart™ Buffer Tips for Maximizing Ligation Efficiencies 应用实例 Breaking through the Limitations of Golden Gate Assembly ...
T4 多聚核苷酸激酶能够催化 ATP 的γ-位磷酸基团转移到寡核苷酸链(双链或单链 DNA 或 RNA)的 5´-羟基末端以及 3´-单磷酸核苷上。T4 多聚核苷酸激酶还具有 3´ 磷酸酶活性,将 3´-磷酸基团从寡核苷酸的 3´ 磷酸末端、脱氧 3´-单磷酸核苷和脱氧 3´-二磷酸核苷上水解掉(1)。
H om e ?Ligation P rotocol with T4 D N A Ligase (M0202) Ligation Protocol with T4 DNA Ligase (M0202) Protocol 1. Set up the following reaction in a microcentrifuge tube on ice. (T4 DNA Ligase should be added last. Note that the table show s a ligation using a molar ratio of 1...
Protocol 1.Setupthefollowingreactioninamicrocentrifugetubeonice. (T4DNALigaseshouldbeaddedlast.Notethatthetableshowsaligationusingamolarratioof1:3vectortoinsert.) COMPONENT20μlREACTION 10XT4DNALigaseBuffer*2μl VectorDNA(3kb)50ng(0.025pmol) InsertDNA(1kb)50ng(0.076pmol) Nuclease-freewaterto20μl ...
T4 DNA Ligase说明书 T4 DNA Ligase, 5 u/µl #EL0011 1000 u Lot Expiry Date Store at -20°C www.fermentas.com Ordering Information T4 DNA Ligase, 5 u*/μl Component #EL0014 #EL0011 #EL0012 T4 DNA Ligase, 5 u/µl 200 u 1000 u 5x1000 u 10X T4 DNA Ligase Buffer 0.5 ...