突变信息查询网站：http://signal.salk.edu/cgi-bin/tdnaexpress/引物设计网站：http://signal.salk.edu/tdnaprimers.2.html 利用突变信息查询网站查询拟南芥T-DNA插入信息，其过程如下：STEP 01打开网站；STEP 02在箭头指的红色框内输入想要查询的基因号，比如：AT2G***，输入之后点Search，得到如下页面，箭头指...
假如是自己设计,一般进行鉴定的引物距离T-DNA插入的位置300bp以上为宜。 自己设计的话需要在http://signal.salk.edu/cgi-bin/tdnaexpress查看插入方向 箭头方向为插入方向 查看插入位点 SALK没有的需要查询TAIR,得到插入位点与方向 https://www.arabidopsis.org 进入网站 http://www.ncbi.nlm.nih.gov/tools/prime...
10、/打开的页面如下:打开的页面如下:点击点击T-DNA Express:输入基因名称输入基因名称AT1G69120,点击,点击search:82012.11.28查找基因查找基因AT1G69120从上述窗口中可以获得很多从上述窗口中可以获得很多不同的突变体,专找不同的突变体,专找SALK_冠名的那些,获得冠名的那些,获得比较方便且便宜,有利于插比较方便且...
1 实验设计的思路和原理 经典遗传学是从生物的性状、表型到遗传物质来研 究生命的发生与发展规律。反向遗传学则是是在获得生物体基因组全部序列的 基础上，通过对靶基因进行必要的加工和修饰，如定点突变、基因插入\缺失、基因置换等，再按组成顺序构建含生物体必需元件的修饰基因组，让其装配出具有生命活性的个体，...
Disarming mutations of tmr and tms T-DNA genes are disclosed which are useful in an Agrobacterium tumefaciens Ti plasmid-based plant transformation vector. As disarmed T-DNA is compatible with standard plant regeneration protocols, such a vector may be used to introduce a plant-expressible foreign...
Tobacco plants made transgenic to express the wild type tobacco mosaic virus (TMV) 183-kDa replicase gene were not resistant to TMV. However, transgenic pl... J Donson,CM Kearney,TH Turpen,... - Molecular plant-microbe interactions : MPMI 被引量: 163发表: 1993年 ...
Disarming mutations of tmr and tms T-DNA genes are disclosed which are useful in an Agrobacterium tumefaciens Ti plasmid-based plant transformation vector. As disarmed T-DNA is compatible with standard plant regeneration protocols, such a vector may be used to introduce a plant-expressible foreign...
Thus, transgenic plants have been generated that express such desirable properties as herbicide tolerance, viral and insect resistance, modified growth and developmental characteristics, and altered nutritional value. T-DNA transfer and integration into the plant genome requires interaction of the associated...
点击突变体编号后进入的网页 http://www.arabidopsis.org 进入网站http://signal.salk.edu/cgi-bin/tdnaexpress查找基因突变体及插入方向 实验设计 T-DNA方向 3’引物 基因方向 5’引物 引物设计 •基因上面引物的设计可以通过下面提供的拟南芥网站http://signal.salk.edu/tdnaprimers.html进行自动生成，也可以...
T-DNA Primer Design ( Powered by GEBD )Please use the backup page served by AtTA, if the tdnaexpress server is down.The new T-DNA Primer Design Tool is now powered by Genome Express Browser Server (GEBD). The new tool can return the primers faster, and also give the insertion ...