竞品1-4代表含血清添加物的培养基,包括(排名不分先后)X-VIVO™ 15 + 血清, CTS™ OpTmizer™ T Cell Expansion SFM + 血清, RPMI 1640 +血清, and IMDM + 血清。每个带误差线的柱形图代表mean ± S.E.M 。 图3. ImmunoCult™-XF T细胞扩增培养基维持培养之前相同的CD4+和CD8+细胞比例...
T. et al. T cells maintain an exhausted phenotype after antigen withdrawal and population reexpansion. Nat. Immunol. 14, 603–610 (2013). Article CAS PubMed Google Scholar Im, S. J. et al. Defining CD8+ T cells that provide the proliferative burst after PD-1 therapy. Nature 537, ...
Synthetic antigen-presenting cells (APCs) are used to mediate scalable ex vivo T-cell expansion for adoptive cell therapy. Recently, we developed APC-mimetic scaffolds (APC-ms), which present signals to T cells in a physiological manner to mediate rapid and controlled T-cell expansion. APC-ms...
Cell expansion is typically a long and labor-intensive step in CAR-T cell manufacture. The Xuri Cell Expansion System (CES) W25 semiautomates this step while functionally closing the process. Cells for autologous or allogeneic cell therapies are cultured inside a single-use Xuri Cellbag bioreactor...
Collectively, these data indicated that dietary serine restriction could impact antigen-specific T cell expansion in vivo. One consequence of impaired expansion of Teff cells is a reduced memory T cell pool and reduced T cell responses and pathogen clearance upon re-challenge (Harty and Badovinac,...
In the HLA-matched setting, minor histocompatibility antigens (MiHA) are perceived as the main stimulus for donor alloreactive T-cell activation and expansion in vivo, resulting in GVHD and graft-vs-leukemia (GVL) effects 3, 4. Although the antigenic disparity between HLA-identical siblings ...
Vaccine-based expansion of T cells is one approach to enhance the graft-versus-tumor effect of allogeneic bone marrow transplantation (BMT), but the complex immunobiology of the allogeneic environment on responses to tumor vaccines has n... CM Capitini,S Herby,M Milliron,... - 《Blood》 被...
After intrapleural T cell administration, prompt in vivo antigen-induced T cell activation allowed robust CAR T cell expansion and effector differentiation, resulting in enhanced antitumor efficacy and functional T cell persistence for 200 days. Regional T cell administration also promoted efficient ...
Short-interval sequential CAR-T cell infusion may enhance prior CAR-T cell expansion to augment anti-lymphoma response in B-NHL. Front Oncol. 2021;11:640166. Article PubMed PubMed Central Google Scholar Pan J, Zuo S, Deng B, Xu X, Li C, Zheng Q, et al. Sequential CD19-22 CAR ...
Interrupting CD28 costimulation before antigen rechallenge affects CD8+ T-cell expansion and effector functions during secondary response in mice. Eur. J. Immunol. 46, 1644–1655 (2016). Article PubMed CAS Google Scholar Sallusto, F., Geginat, J. & Lanzavecchia, A. Central memory and ...