Overview of the described computational methods and their coverage of the typical cytometry data analysis workflow. t-SNE和各种大数据分析已经成为非常重要的工具,在各类杂志,高低分文章中已经屡见不鲜,小编本周随意整理了汇集十数篇含有T-sne等大数据处理的发表文献,让您先一睹为快!点击图片可以直接阅读文献呦...
As an in-process control during manufacture, t-SNE analysis can also identify lines that deviate from accepted parameters, providing a go/no go point in a manufacturing run that would otherwise fail to meet final product release criteria. The visualisation of cytokine response within VST cell ...
we depleted intratumoral macrophages using Cl2MDA-liposomes or an antibody against the CSF1 receptor (αCSF1R)24,25prior to local IR (Fig.2a). Flow cytometry analysis confirmed that both Cl2MDA-liposomes and αCSF1R significantly depleted intratumoral CD11b+F4/80+macrophages...
Additionally, the flow cytometric data was randomly down-sampled to 2,500 CD45 + CD3 + events for each donor and analyzed using t-distributed stochastic neighbor embedding (t-SNE)35 with the Rtsne package36. Flow cytometry validation of gene expression Based on the results of RNA ...
Cells were harvested after 10 days post-stimulation; quantification of peptide-specific T cell immune response intensity was conducted with the IFN-γ ELISA and flow cytometry assay. T cell response analysis by IFN-γ ELISA assay IFN-γ secretion of T cells was measured by ELISA using human ...
(n = 35 donors).dRepresentative flow cytometry of BTLA+CD4+T-cell subsets and BTLA-CD4+T-cell subsets.eDifferentiation phenotypes of BTLA+CD4+T cells in NC (n = 26 donors), CHB (n = 17 donors), and HBV-ACLF patients (n = 13 donors). Compared with BTLA-CD4+...
After overnight co-culture (18-24 h), the cells were harvested and transferred from the ELISPOT plate into a round-bottom 96-well plate for flow cytometry staining and analysis. Then the ELISPOT plates were washed 6x with PBS containing 0.05% Tween 20 (PBS-T, Sigma-Aldrich), and ...
Pathway analysis Generally applicable gene set enrichment (GAGE) method was used [36] based on the log2 fold changes from the single-cell differential expression analysis. Flow cytometry Murine forebrain cells were isolated and stained with fluorescence-conjugated antibodies. Briefly, mice were anestheti...
a, t-SNE map was calculated with all SARS-CoV-2-specific CD8+ T-cell data during (6 dps) and after (79 dps) infection (tet-t-SNE). Wanderlust trajectory analysis was performed on virus-specific CD8+ T cells. Wanderlust trajectory is indicated by heatmap colorization on the tet-t-SNE...
After 60 years of intense fundamental research into T cell-mediated cytotoxicity, we have gained a detailed knowledge of the cells involved, specific recognition mechanisms and post-recognition perforin–granzyme-based and FAS-based molecular mechanisms.