T-SPOT.TB是以EL ISPOT 技术检测结核感染的新方法, ELISPOT 技术是近20年发展起来的检测抗原特异性T细胞的技术, 是目前最敏感的检测抗原特异性T细胞的方法之一, 可以检测出1 /100 000~1 /50 000外周血单个核细胞(peripheral blood mononuclear cells, PBMCs)中经某种抗原刺激后释放某种细胞因子的细胞, 经过酶联...
T-SPOT.TB是以EL ISPOT 技术检测结核感染的新方法, ELISPOT 技术是近20年发展起来的检测抗原特异性T细胞的技术, 是目前最敏感的检测抗原特异性T细胞的方法之一, 可以检测出1 /100 000~1 /50 000外周血单个核细胞(peripheral blood mononuclear cells, PBMCs)中经某种抗原刺激后释放某种细胞因子的细胞, 经过酶联...
we were able to confirm the presence of CD8+TRMs in multiple tissues using samples from the previous study22. There were abundant Gag-specific CD8+T cells in blood, spleen and multiple parts of
It also remains a topic of discussion how cT n forms (either intact or fragments) are cleared from the blood circulation, but it is thought that smaller proteins pass through the glomerular membrane for clearance.20 After an acute myocardial infarction, however, extrarenal clearance turned out to...
To further investigate the differentiation efficiency of iHECs into iT cells, we sorted single iHECs into individual wells (24 well-plates) pre-seeded with OP9-DL1 feeder cells (Fig.3a). After ten-day co-culture, over 15% individual iHECs formed blood colonies (76/384 wells) (Fig.3b), ...
Normal human peripheral blood cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD8a Monoclonal Antibody, eFluor 450 (Product # 48-0087-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control, P...
whether they represent different types of disease-specific fragments. It also remains a topic of discussion how cT n forms (either intact or fragments) are cleared from the blood circulation, but it is thought that smaller proteins pass through the glomerular membrane for clearance.20 After an ...
lergybythelymphocytestimulationtestwiththeMTTassayOliverRT.Anewapproachformeasure-mentofcytotoxicityusingcolorimetricassay.JImmunolMeth-odsetal.Themonitoringbiomarkerforim-munefunctionoflymphocytesintheelderly.MechAgeingDevetal.Blooddonorde-riveddendriticcellsandcytotoxicTcellsforspecificfusion-geneadoptiveimmunotherapy ...
Protein bands were detected using the Bio-Rad ChemiDoc™ (Hercules, CA, USA). GAPDH was used as an internal control. Cytokine assay Spleen cells were activated with plate-bound anti-CD3 (5 µg/ml) plus soluble anti-CD28 (2 µg/ml, BD Biosci- ence) and stimulated with ...
Interviewer: OK, thank you. We know that the initial CAR-T cell therapy is mainly used in blood tumors and has achieved good results, but the treatment of solid tumors is still under research. Do you think the effect of CAR-...