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Chromatographic separation was achieved using a Waters Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 μm) (Milford, Massachusetts, USA) equipped with a Waters VanGuard Acquity C18 guard column (2.1 × 5 mm, 1.7 μm). The binary mobile phase system consisted of phase A (0.1% AA in ...
The analysis was carried out in elution extrusion mode, using the biphasic solvent system n-Heptane/EtOAc/MeOH/H2O (1:3:1:3, v/v/v/v), the upper phase of which served as the stationary phase, while its lower phase served as the mobile one. In the first step, the stationary phase ...
It is our intention to subsequently re-address this shortcoming in future xenograft mouse model experiments by utilizing a fused microPET/CT imaging platform in all of the xenograft mice. As a last notable point of discussion, some may contend that the lack of specific localization of 18F-FDG ...
The isola- tion of drug and its metabolite was performed on Waters Symmetry C18 Column (150×4.6 mm; 5.0 μm, Waters Corporation, Milford, MA, USA). The column temperature was maintained at 35°C. UV detector wavelength was set at 246 nm. The mobile phase contained of 55% water formic...
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Mobile phase A was 0.1% FA in water, B was 0.1% FA in ACN. The flow rate was 0.2 mL/min, and the column temperature was 40 °C. An auxiliary pump delivered a lockmass solution (100 fmol/µL (GLu1)-fibrinopeptide B (GFP) in 50:50 ACN/water containing 0.1% FA) for mass...
Figures 3 and 4 show the PDA and MS chromatograms from 1 min- ute and 32 hours. The vertical axes have been linked in both cases.Marian TwohigDarcy ShaveWaters CorporationMilfordRobert S. Plumb
THF stabilized with 2,6-tert-butyl-4-methylphenol (BHT) was used as the mobile phase, at a flow rate of 0.5 mL/min at 40 ◦C. Three methods for estimation of MM were applied. Firstly, MM were obtained by the refractometric detection (polystyrene standards). Secondly, a combination of...
Fatty acids were separated on an Acquity UPLC HSS T3 column (100 × 2.1 mm; 100Å; 1.8 μm) (Waters, Milford, MA, USA). The mobile phases consisted of (A) 60:40 (v/v) acetonitrile:water with 10mM ammonium acetate and (B) 90:10 (v/v) isopropanol:acetonitrile with 10mM ammonium...