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(IDT; Coralville, IA, USA). This fragment also contained the upstream bases present between the Age I and Nco I sites within the SFG retroviral vector and was ligated into the unique Age I and Xho I restriction sites in the SFG vector plasmid (all restriction endonucleases were from New ...
In 2011, a bunch of fun-haters who apparently don't like refreshment on a hot day or having their lawn mowed for them, shut down a lemonade stand run by kids during RAGBRAI's visit to Coralville. They reportedly did so because the kids didn't have a "license" or "permit" to run t...