1. Protocol for SALK T-DNA primer design Note:N - Difference of the actual insertion site and the flanking sequence position, usually 0 - 300 bases MaxN - Maximum difference of the actual insertion site and the sequence, default 300 bps pZone - Regions used to pick up primers, default ...
1. Protocol for SALK T-DNA primer design Note: N - Difference of the actual insertion site and the flanking sequence position, usually 0 - 300 bases MaxN - Maximum difference of the actual insertion site and the sequence, default 300 bps pZone - Regions used to pick up primers, default...
2. SALK T-DNA verification primer design The program will pick up LP and RP for you. Its product size is around 900-1100 bps. If you want to confirm whether the design is right, simply cut and paste the LP and RP sequences together (you can cut/paste whole returned primer info line...
点击SIGnAL T-DNA Verification Primer Design 将SALK号输入 设计好了,记录下LP与RP的序列及退火温度。 假如是自己设计,一般进行鉴定的引物距离T-DNA插入的位置300bp以上为宜。 自己设计的话需要在http://signal.salk.edu/cgi-bin/tdnaexpress查看插入方向 箭头方向为插入方向 查看插入位点 SALK没有的需要查询TAIR,...
LOGO模式植物拟南芥模式植物拟南芥TDNATDNA插插入突变体的鉴定入突变体的鉴定2012.11.282012.11.2812012.11.28目录目录拟南芥的栽培拟南芥的栽培拟南芥拟南芥TDNATDNA插入突变体插入突变体的的PCRPC
聚合酶AmpliTaq Gold DNA 聚合酶 反应速度标准 适用于(设备)7000 系统、7300 系统、7500系统、7700 系统、7900HT 系统、Applied Biosystems StepOnePlus™ 快速实时 PCR 系统、StepOne™、标准模式、StepOnePlus™、标准模式 参比荧光染料ROX(预混) 产品线RNA-to-CT™,TaqMan™ ...
Briefly, for each sample, barcoded full-length complementary DNA (cDNA) was generated with Maxima RT polymerase (EP0742, Thermo) using oligonucleotide-dT primer-containing barcodes, unique molecular identifiers (UMIs) and an adaptor. The addition of a template switch oligonucleotide resulted in ...
1 知乎:三引物法鉴定T-DNA插入突变体 https://zhuanlan.zhihu.com/p/377200556 2 LP RP 引物设计网站: T-DNA Primer Design http://signal.salk.edu/tdnaprimers.2.html 3 简书: 拟南芥突变体信息查询 https://www.jianshu.com/p/db695e0756ba...
Positive cells were sorted into 200 μL lysis buffer (10 mM Tris-HCl, pH 8.8, 5 mM EDTA, 0.2% SDS, 0.2 M NaCl, 25 μg/mL Proteinase K (Calbiochem)) for subsequent genome DNA isolation. Sanger sequencing and analysis using EditR PCR primers about 150bp upstream and ...
由于抗原受体基因的多态性(包含相关DNA序列的异质性群体),很难设计出一套引物可以把基因重排V-J区的侧翼序列全部包括在内,N区的多样性及体细胞突变更增加了该区段序列的多变性。因此,试剂盒设计了多管扩增Mix,定位多个FR区,旨在检测出绝大多数的基因重排。 在扩增结束后,可以通过聚丙烯酰胺电泳或毛细管电泳的方法...