cruzi OligoC-TesT, a simple and standardized dipstick format for detection of PCR amplified T. cruzi DNA. The specificity and sensitivity of the assay were evaluated on blood samples from 60 Chagas non-endemic and 48 endemic control persons and on biological samples from 33 patients, 7 ...
This result is consistent with the effector function of Tem cells, which, although short-lived (61), contribute to IFN-γ production and protection against chronic intracellular parasitic infections with Plasmodium chabaudi and Trypanosoma cruzi in the mouse (62–64). Taken together, our data ...
2.根据权利要求1所述的方法,其特征在于通过TTV序列的扩增、测序或杂交来测量所述TTV载量,优选通过扩增,更优选通过实时PCR。 3.根据权利要求1或2所述的方法,其特征在于所述生物样品是全血、血浆或血清样品。 4.根据权利要求1至3中任一项所述的方法,其特征在于所述步骤b)中的确定包括将a)中测量的TTV载量与参考...
Analyses for miRNA-181a were performed by TaqMan real-time qRT-PCR assay kit (has-miR-181a; Applied Biosystems) on the ABI-Prism 7900 system (Applied Biosystems) and based on the manufacturer’s technical recommendation, snoRNA202 was used as the internal control. In each set, the test ...
Analyses for miRNA-181a were performed by TaqMan real-time qRT-PCR assay kit (has-miR-181a; Applied Biosystems) on the ABI-Prism 7900 system (Applied Biosystems) and based on the manufacturer’s technical recommendation, snoRNA202 was used as the internal control. In each set, the test ...
RT-PCR analysis showed that the level of PTX3 mRNA was significantly increased at the cutaneous site following infection (Figure 1A). Likewise, the expression of PTX3 protein (Figures 1B and 1C) was higher at the site of L. major infection compared to uninfected sites, and the expression...
cruzi, concurrent with the release of anti-inflammatory cytokines during macrophage infection, and dramatically alters macrophage morphology to promote the creation of migratory macrophages.To what extent the ageusia research literature has evolved remains a question yet to be addressed. Web of Science's...
In animal models, NOS2-producing cells have been reported to infiltrate the myocardium of α-MyHC/CFA–immunized mice and on infection with Coxsackievirus or Trypanosoma cruzi. In EAM, NOS2 production entirely depends on IFN-γ. NOS2-dependent nitric oxide has been shown to functionally eliminate...
Real-time PCR was performed on a QuantStudio 7 Flex or QuantStudio 7 Pro system (Thermo Fisher Scientific) using QuantStudio software version 1.3 or Design and Analysis software 2.6.0, respectively. Gene expression of Cdkn1b and/or 18S or Hprt was determined as endogenous controls, and ΔCt ...
cruzi (Bittencourt, 1992; Torrico et al., 2004, 2006) and malaria (Malhotra et al., 2006; Okafor et al., 2006). Show abstract An Overview of Peripheral Blood Mononuclear Cells as a Model for Immunological Research of Toxoplasma gondii and Other Apicomplexan Parasites 2019, Frontiers in ...