T cell receptor transgenic (TCR-Tg) mice specific for the arthritogenic 5/4E8 epitope in the G1 domain of cartilage proteoglycan were generated and back-crossed into arthritis-prone BALB/c background. Although more than 90% of CD4+ T cells of all TCR-Tg lines were 5/4E8-specific, one...
1.2 T cell and its subsets T cells play a crucial role in the eradication of pathogens and the scrutiny of pathological cells. T cells are of two major subsets founded on their unique T cell receptor (TCR) chain, cytokine expression profile, and co-receptor expression. Based on TCR, they...
This system relies on P14 T cell receptor (TCR) transgenic CD8 T cells, which recognize the gp33 epitope of the commonly used in mouse infection models lymphocytic choriomeningitis virus (LCMV; Supplementary Fig. 1A). In a typical experiment, naive P14 T cells are obtained from transgenic ...
Tcf1 Protein Expression Is Progressively Decreased during Lung TRM Cell Development To determine the expression kinetics of Tcf1 in lung CD8+ TRM cells after infection, we first generated the T cell receptor (TCR) transgenic P14 CD8+ T cell “chimeric mice” by adoptively transferring 104 P14 ...
(adapted fromBioRender.com).f, Glutarate levels in naive and 72-h activated mouse CD8+T cells. Two-tailed pairedt-test;n = 3.g, GCDH protein copy number in naive or activated CD8+T cells from P14 transgenic mice. Two-tailed unpairedt-test;n = 3. Data from the ImmPRes ...
cells from P14 TCR transgenic mice (specific for the LCMV CD8 epitope, GP33–41). We confirmed the efficient deletion (~80%) of Ptpn2 in naive P14 CD8+ T cells using the tracking of indels by decomposition (TIDE) assay29 (Fig. 1b). To evaluate cellintrinsic functions of PTPN2 in ...
Recent studies have shown that CD32/CD8a/CD28/CD3ζ chimeric receptor cells directly kill breast cancer cells, suggesting the existence of cell surface myeloid FcγR alternative ligands (ALs). Here, we investigated the metabolism, ALs, cytotoxicity, and immunoregulatory functions of CD64/CD28/CD3ζ...
PD-1 suppresses T cell spreading on pMHC surface We first examined T cell spreading on coverslips functionalized by pMHC with or without PD-L1 (Fig. 1). CD8+ T cells from PDCD1−/− P14 TCR-transgenic mice re-expressing PD-1 or vehicle spread on gp33:H2-Db (but not BSA) surface...
Thus, costimulation can partially repair poor activation of transgenic CD8+ T cells by H-Y antigen due to low avidity/affinity of TCR-H-Y antigen/Db interaction and/or elevated threshold of CD8+ T cell activation. 展开 学位级别: Ph.D. 学位年度: 1999 ...
The present invention is directed to a functional T cell receptor (TCR) fusion protein (TFP) recognizing and binding to at least one MHC-presented epitope, and containing at least o