「英文标题:」 ImmuCellAI: A Unique Method for Comprehensive T-Cell Subsets Abundance Prediction and its Application in Cancer Immunotherapy 「网址:」 https://bioinfo.life.hust.edu.cn/web/ImmuCellAI/ 「期刊:」《「Advanced Science」》 「发表时间:」 2020 年02月11日 「研究领域:」 肿瘤浸润 「DO...
Tumour mutational burden (TMB) predicts immunotherapy outcome in non-small cell lung cancer (NSCLC), consistent with immune recognition of tumour neoantigens. However, persistent antigen exposure is detrimental for T cell function. How TMB affects CD4 and CD8 T cell differentiation in untreated tumour...
Relative cell-type enrichment of CD8+ T-cells was estimated from the total RNA sequencing data using xCell in R(v.1.1.0) [40]. xCell is a gene expression signature-based approach to determine cell-type enrichment from bulk tissue RNA expression data, used here to explore the enrichment of...
Ubash3b encodes the protein Sts-1 (suppressor of T cell signaling-1), a phosphatase shown to be a potent negative regulator of TCR signaling by inhibiting Zap70.16,17 Ubash3b expression is increased in CD8+ TEFF compared with naive cells (ImmGen RNA-seq dataset), suggesting that Sts-1 ...
eliminating the need for bridging therapy. T-cell subsets were assessed in the apheresis starting material and final CAR-T product. There was a higher proportion of early phenotypes of CD4+ and CD8+ CAR T cells (naive/stem cell memory and...
Odc-ΔT mice displayed comparable CD4+ and CD8+ T cell frequencies and numbers in spleen, and naive and memory cells within the CD4+ T cell compartment, relative to wild-type (WT) littermates (Figures S1E and S1F). Importantly, Odc−/− CD4+ T cells had reduced intracellular ...
estimated to have a low level of exhaustion, perhaps due to latent viral infections, for example from Epstein-Barr Virus, where up to 2.5% of CD8+ T cells are specific to EBV in healthy individuals62,64. In general, the xCell method struggles with these T cell isolates, exhibiting ...
CD8+T cells and tumour cells, it has been increasingly recognized that different CD8+T-cell distributions in the tumour microenvironment (TME) may elicit different responses to immunotherapy8,9. Several studies have also shown that the numbers of tumour-infiltrating CD8+T cells are associated with...
Neo-mDC-induced T cells exhibit a continuum of transcriptional differentiation states ranging from naive-like to terminal effector T cells To further characterize vaccine-induced T cells at the population and clonal level, CD8+ tetramer+ cells were sorted ex vivo from on-treatment and post-treatment...
Additionally, CD44+PD1+CD8+ T cells maintain a Ly108+TIM3− Tex_prog phenotype and do not progress to Tex_term cells, even when they are trapped in the tdLN with FTY720 administration (Figure S1C). We next examined the evolution of T cell exhaustion within the tumor over time. We ...