. Phorbol 12-myristate13-acetate (PMA) (Catalog #79346) and Ionomycin (Catalog #I9657) were purchased from Sigma-Aldrich (St. Louis, MO). Cell lines Mouse tumor cell lines MC38 (colon adenocarcinoma), B16F10 and B16-OVA (melanoma), and Jurkat cells (human T-cell lymphoblast) were ...
Fig. 6: M2 blocks T cell activation mediated by hB7.1/2. aPurified human T cells were co-cultured with 293T-hB7.1/2 cells on anti-human CD3 pre-coated plates or stimulated with PMA/Ionomycin. The cells were cultured in the medium supplemented with wild-type MPXV M2 (0, 0.1, or 1...
Gp41 TMD does not inhibit T-cell activation induced by PMA/ionomycin.Tomer, CohenShmuel, Jaffe CohenNiv, AntonovskyIrun, R. CohenYechiel, Shai
PMA SigmaAldrich Cat# P-8139 Ionomycin SigmaAldrich Cat# I-0634 1-step NBT/BCIP Substrate Thermofisher Cat# 34042 CellTrace CFSE Cell Proliferation Thermofisher Cat# C34554 CellTrace Far Red Cell Proliferation Thermofisher Cat# C45571 Golgi plug BD Biosciences Cat# 5550291 C646 SigmaAldrich Cat...
对于细胞内细胞因子染色,用50 ng ml^-1 phorbol 12-myristate 13-acetate(PMA)和 0.5 μg ml-1 ionomycin(ION)在37 °C 下刺激 5 × 10^4 个T细胞2 h;随后,添加 100 µg ml−1 brefeldin A (Merck蛋白转运抑制剂) 在 37 °C 下再持续 2 h。根据制造商的说明,...
not result in significant SARS-CoV-2-specific T-cell responses, due to the immunodominance of NP366influenza T-cell epitope. However, side-by-side stimulation of peripheral blood mononuclear cells (PBMCs) of COVID-19 convalescents with recombinant viruses and LAIV vector demonstrated activation ...
Also at this time, positive control wells were treated with 1x Cell Stimulation Mix (eBioscience) containing a cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin. All cells were returned to the incubator for an additional 4 hours. After 24 hours total stimulation, cells from ...
atthetimepointsindicated,stimulatedwithPMA/ ionomycin,andyzedbyICSandBDCBA.Datafromthe Th17polarizationisshownasanexampleofICS,andall threeconditionsareshownforBDCBA.Combiningthese techniques,similartrendswereobservedwhencomparing thereaseinnumberofcellpressingthecytoko thetotalamountofsecretedcytokines. IFN-IL...
(right) display frequencies of IFN-γ, IL-2, and TNF-α-expressing cells on CD4+T cells induced by PMA/ionomycin for 6 h in the presence of control shRNA and BTLA shRNA (n = 6 donors).eExpression of CD25, CD38, and CD69 on CD4+T cells activated by anti-CD3/CD28 for ...
(Supplementary Fig.3b). Moreover, SARS-CoV-2 viral load positively correlated with the overall capacity of CD4+T cells to secrete IL-4 in response to TCR independent unspecific activation with PMA/ionomycin (PMA/Io) (Supplementary Fig.3c). In contrast, the expression of IL-10, a proto...