图1. T Cell TransAct 对 T 细胞的激活效率与微米级磁珠的激活试剂相当。分别用 T Cell TransAct 和其他供应商基于磁珠的激活试剂激活 T 细胞 2 天后,流式细胞术分析活化标志物 CD69 和 CD25 的表达,结果显示两组激活试剂的活化效率相当。 图2. T 细胞分别在 TexMACS 培养基中用 T Cell TransAct 激活,或...
T Cell TransAct™ 有效激活 T 细胞且能快速扩增 图1. T Cell TransAct 对 T 细胞的激活效率与微米级磁珠的激活试剂相当。分别用 T Cell TransAct 和其他供应商基于磁珠的激活试剂激活 T 细胞 2 天后,流式细胞术分析活化标志物 CD69 和 CD25 的表达,结果显示两组激活试剂的活化效率相当。 图2. T 细胞分...
Richter GH, Mollweide A, Hanewinkel K et al (2009) CD25 blockade protects T cells from activation-induced cell death (AICD) via maintenance of TOSO expression. Scand J Immunol 70:206-215Richter GH, Mollweide A, Hanewinkel K, Zobywalski C, Burdach S. CD25 blockade protects T cells from...
We have, therefore, expanded our earlier work by using a panel of different T cell activation markers (CD25, CD26, CD40L, CD69). The study is based on 58 culprit lesions from patients who underwent coronary atherectomy. There were four groups of patients: chronic stable angina ( n=13)...
(cytotoxic T lymphocyte-associated antigen-4,CTLA-4)、淋巴细胞活化基因 3 蛋白(lymphocyte activation gene-3,LAG-3)等抑制受体直接接触靶细胞或分泌抗炎细胞因子 IL-10、IL-35 和 TGF-β 来抑制免疫反应,Treg 细胞也通过表面 ...
cells after TCR-mediated stimulation from 24 to 120 h (Fig.5b, upper), and with each successive round of T cell proliferation, while CD25 was expressed similarly, independent of cell division (Fig.5b, lower). These results establish NME1 expression as a marker of T cell activation, ...
since during the immune response the activated T lymphocytes undergo rapid expansion to target and attack the tumor. T cell activation can be measured in multiple ways, including proliferation, up-regulation of activation markers (e.g., IL2RA/CD25), and production of effector cytokines such as ...
增强子对 T 细胞状态的信号依赖性功能调节作用进一步体现在 IL2RA 基因(编码 CD25,高亲和力 IL-2 受体的一个亚基)上,该基因中一个内含子 DNA 元件的缺失导致了初始 T 细胞从诱导的 Treg 细胞极化为体内促炎的Th17 细胞[12]。另一项研究表明,PDCD1基因上游增强子元件的缺失降低了抗原特异性CD8+T 细胞中活化...
引人注目的是,添加富马酸盐而非其他 TCA 循环代谢物导致 CD8+ T 细胞中 CD69、CD25 和 CD44 的表达减少。 用富马酸或其酯类富马酸二甲酯 (DMF) 和富马酸单甲酯 (MMF) 处理以剂量依赖性方式减弱 CD8+ T 细胞活化(图 2H)。 同样,通过使用 FH-IN-1(一种 FH 抑制剂 (FHi))积累内在富马酸盐,以浓...
CD4+ CD25+ T Cells Regulate Vaccine-Generated Primary and Memory CD8+ T-Cell Responses against Herpes Simplex Virus Type 1 It has become evident that naturally occurring CD25(+) regulatory T cells (T(reg) cells) not only influence self-antigen specific but also dampen foreign a... FN Toka...