SLC7A11是一种多次跨膜蛋白,可导入胱氨酸以进行GSH生物合成和抗氧化防御,SLC3A2是一种单跨膜蛋白,具有维持SLC7A11蛋白的稳定性并向胞膜运输的作用,其功能不仅限于胱氨酸转运,还转运多种氨基酸蛋白[26],二者是合成GSH的重要参与蛋白[27]。激活System Xc-可以促进细胞内胱氨酸的摄取并将其还原为半胱氨酸,半胱氨酸与谷氨酸和甘氨酸经过谷氨酸半胱氨酸连接酶(...
579 Bilirubin Upregulates Gene Expression of Slc7A11 and Increases Cystine Uptake Mediated by System Xc- in Sh-Sy5Y Neuroblastoma CellsPediatric Research publishes original papers, invited reviews, and commentaries on the etiologies of diseases of children and disorders of development, extending from ...
2.9Western blotting检测胰腺组织相关蛋白表达 取胰腺组织放入装有一定比例PIPA裂解液的EP管内,研磨组织后置于冰上裂解,12 000 r/min离心15 min,取上清于−20℃保存;检测蛋白浓度,蛋白样品经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳,转至PVDF...
System xc- was eliminated by mutations in the gene slc7a11, which encodes xCT, the core transporter protein required for system xc- function. We analyzed two motor behavior tasks and two tasks related to exploratory behavior and working memory [28]. To ensure that phenotypes could be ...
the system xc−inhibitor Sorafenib or Imidazole ketone erastin effectively inhibited the growth of tamoxifen-resistant breast cells in vitro and in vivo. In conclusion, our data reveal that targeting estrogen-regulated SLC7A11 and SLC3A2 enhances ferroptosis in ER+ breast cancer, offering a novel...
579 BILIRUBIN UPREGULATES GENE EXPRESSION OF SLC7A11 AND INCREASES CYSTINE UPTAKE MEDIATED BY SYSTEM XC- IN SH-SY5Y NEUROBLASTOMA CELLS P.J. Giraudi, C. Bellarosa, C. Coda-Zabetta, C. Tiribelli Liver Research Center (CSF), Trieste, Italy Background and aims: Severe neonatal ...
The equimolar transport of these 2 amino acids is mediated by the cell surface antiporter, system xc− [8], [9]. Neurons selected for resistance to oxidative stress express higher levels of the light chain component of system xc−, xCT [10], which is encoded by the SLC7A11 gene and...
IKE (500 nM; 5-360 min) 增加SUDHL-6细胞中系统xc组分SLC7A11、前列腺素内过氧化物合酶2 (PTGS2)和ChaC谷胱甘肽特异性γ-谷氨酰环化转移酶1 (CHAC1)的表达[1]。IKE (500 nM和1 μM) 可改变 SUDHL6 细胞中溶血磷脂酰胆碱 (LPC)、磷脂酰胆碱 (PC)、磷脂酰乙醇胺 (PE) 和甘油三酯 (TAG) 等 ...
与RA组比较,铁死亡抑制剂组PBMC内Fe 2+浓度和ROS水平降低,SLC7A11,GPX4蛋白和mRNA的表达升高,Nrf2蛋白的表达升高,PBMC上清液中IL-6的含量降低,TNF-α的含量升高,差异均有统计学意义.与RA组比较,铁死亡诱导剂组PBMC内ROS水平升高,SLC7A11蛋白和mRNA的表达降低,Nrf2蛋白的表达降低,GPX4蛋白的表达升高,PBMC上清液中...
[25]. In the latter, cysteine originates from the reduction of cystine transported into the cell by cystine/glutamate antiporter (system Xc − , xCT) [26]. The system Xc−, a dimer consisting of SLC7A11 and SLC3A2, exchanges glutamate (Glu) out of the cell and cystine into ...