CDC updates on syphilis testing for the clinical laboratoryWheeler, SarahClinical Laboratory News
The rise in syphilis rates in 2009 (Fig. 1) are likely reflective in part to a change in the testing algorithm for syphilis in Calgary from an initial RPR to enzyme immunoassay (EIA). In 2008, the screen- ing test was changed to an EIA resulting in increased identification of latent ...
CDC recommends the use of the RPR-based screening algorithm. When there is a low epidemiologic risk or clinical probability of syphilis, the positive predictive value of an isolated unconfirmed reactive treponemal chemoluminescence test or enzyme immunoassay is low. References Antibiotic Therapy Penicillin...
Results of the laboratory‑based (reference) testing Based on the reference test algorithm, 23.46% (n = 598/2549) of all participants were confirmed HIV positive, and 35.88% of all participants (n = 901/2511) were positive on treponemal reference testing. Of all participants ...
Second, the review did not address the comparative screening accuracy of traditional vs reverse sequence algorithm testing; however, to our knowledge, no studies have compared these 2 testing algorithms in prenatal care. Third, the benefit of penicillin G for the treatment of syphilis is well ...
n Two different algorithms, which combine a treponemal with a nontreponemal test, are used to aid in the diagnosis of syphilis. In recent years, the reverse algorithm has become increasingly utilized compared to the traditional algorithm due to reduced screening labor costs and improved ...
algorithm uses an automated, treponemal test such as enzyme immunoassay (EIA).30Samples that are reactive by EIA are then tested by RPR to assess disease and treatment status.30This technique allows clinical laboratories a rapid syphilis testing.30However, the results of EIA screening and RPR are...
testing, in duplicate, of the same sample and with the same assay before conclusions are drawn [6]. Furthermore, repeat testing may be performed with an alternative assay, either an RDT or an EIA [24,36]. Indeed, the WHO established an HIV diagnostic algorithm that has been adapted in ...
At the laboratory, they test for syphilis using either the traditional or the reverse testing algorithms29. With the reverse testing algorithm, the laboratory screens the serum specimen using a specific treponemal test (TPAb) and confirms the treponemal infection using the rapid plasma reagin test (...
The reverse testing algorithm, which first uses a treponemal test, has advantages (Dunseth et al., 2017) and has been adopted in many guidelines (CDC, 2015, HAS, 2017, Kingston et al., 2016, WHO, 2016). However, in low-income countries, the traditional testing algorithm can be adopted ...