In summary: • The crushed-crystal-cat-litter resin worked as well as a commercial miniprep, followed closely by diatomaceous earth. • Crushing the crystal cat litter is necessary; the column with uncrushed resin didn't yield any DNA. • Powdered beer-bottle glass appears to sheer the ...
Recombinant proteins containing a Fc-tag were purified using Protein A resin (1 ml, HiTrap MabSelect PrismA) at a flow rate of 1 ml/min. The column was washed with 30 column volumes of PBS. Proteins were eluted at pH 3.2 to 3.5 using a 50 mM citric acid buffer. Fractions containing ...
Adding peptide nucleic acid units to an amine terminated peptide nucleic acid oligomer on a solid phase synthetic resin; biosynthesisdoi:US6204326 B1New sub-monomer synthetic methods for the preparation of peptide nucleic acid oligomeric structures are disclosed that provide for the synthesis of both ...
fumed silica, which synthesizes a potassium silicate solution, is given in Equation (1). In addition, the chemical reaction to remove potassium ions from the synthesized potassium silicate solution during an ion exchange process with a strongly acidic cation-exchange resin is expressed in Equations ...
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