PROBLEM TO BE SOLVED: To obtain a new DNA which consists of DNA containing at least one of specific sequences with a specific base number and is useful in the method of synthesizing proteins in eukaryotes, the method of synthesizing proteins in acellular systems by the use of an acellular ...
Protein synthesis is a complex and essentially biological process that occurs in living cells and involves the production of specific proteins. The two main steps in protein synthesis are transcription and translation, which are carried out in four phases: initiation, elongation, termination, and recyc...
Protein synthesisis a costlybiochemical processaccounting for approximately 50% of the cell's energy, which is increased incancer cells[1]. Much ofprotein synthesisregulation occurs at the step ofmRNA translationinitiation. Briefly,40S ribosomal subunitsbind eukaryotic initiation factors (eIFs) 1, ...
The single stranded DNA binding protein RPA plays a pivotal role in DNA replication. The archaeal RPA hosts a WH domain that interacts with the DNA primase and the replicative polymerase PolD. Here the authors provide a molecular understanding of the regulatory activity of archaeal RPA. Markel Ma...
Figure 2. In prokaryotes, multiple RNA polymerases can transcribe a single bacterial gene while numerous ribosomes concurrently translate the mRNA transcripts into polypeptides. In this way, a specific protein can rapidly reach a high concentration in the bacterial cell. ...
Here the sequence of mRNA bases is translated into a sequence of amino acids in a polypeptide chain.(4 protein)这里，mRNA的碱基序列被翻译成肽链里氨基酸的序列。Transfer RNA(tRNA) molecules pick up specific amino acid molecule from the cytoplasm according to their anti-codon triplet ...
-complementary to specific codon in mRNA steps of translation 1. Initiation 2. Elongation 3. Termination ricin potential weapon of bioterrorism derived from castor beans binds to ribosomes disrupts protein synthesis mutation change in DNA sequence ...
12、 in the -CCA sequence at the 3-end of the tRNA. tRNA is said to be charged and the amino is said to be activated. Anticodon pairs with a specific codon on the mRNA,Amino attachment,Amynoacyl-tRNA(氨基酰-tRNA合成酶) synthetases catalyze a two-step reaction. Activation of amino aci...
Viruses use the human host cell translation machinery for their protein translation. Therefore, host-specific slow codons and slow di-codons proportions in the viral host sequences can be used to anticipate the order of translation rates between various viruses of different genera, serotypes, and ...
The ligands were also investigated in [35S]GTPγS functional binding assays to examine G-protein activation via opioid receptors. The effect of the investigated derivates was observed in vivo nociceptive tests. The presence or absence of specific functional groups in the orvinol and thevinol ...