Rheumatoid arthritis (RA) is a chronic and widespread autoimmune disease characterized by progressive synovitis and joint degradation [1, 2], with a prevalence rate of approximately 246.6/100,000 [3]. As one of the most common and disabling diseases encountered in clinical practice, the exact eti...
we tested the possibility of using synovial fluid from patients with RA (RASF) to imitate the RA microenvironment in vitro. LAD2 cells were labeled with Indo-1, and intracellular calcium flux was detected by flow cytometry upon RASF stimulation (Fig.3a). Dynamic monitoring for nearly five minut...
h TPPP3 and RUNX2 immunofluorescence staining within human heterotopic ossification in a hip joint (scale bar in panel: 200 µm). i Quantification of TPPP3+ and RUNX2+ cells within the bone area (n = 3 slides per group). For all graphs, each dot represents a single sample, ...
A fNP-labeled ADSCs or SDSCs residing in the knee joint with NIR-II imaging during 0–12 weeks. B quantification of the fluorescence intensity of fNP-labeled ADSCs or SDSCs in the knee joint. C confocal microscopy of fNP labeled ADSCs or SDSCs in knee at 12 weeks, fNP only at 4 ...
What has remained unclear is how the interzone forms to begin with, and how it acquires its compacted, avascular structure composed of flat cells. The future joint sites in the limb are initially cartilaginous and contiguous with the flanking long bone anlaga; in the autopod region, these ...
In synovitis, characterized by inflammation of the synovial tissue lining the joint, CD68-labeled macrophages play a crucial role in pathological detection and research [34, 35]. Their involvement in the inflammatory response and tissue damage was significant, as they released inflammatory mediators ...
The integration process between scATAC-seq and scRNA-seq SFs labeled the scATAC-seq cells according to 9 SF subpopulations (see above) that were visualized in UMAP space (Fig. 1; Additional file 1: Fig. S3). To identify a robust merged peak set along the SF subpopulations, MACS2 [41] ...
portable microfluidic control instrument to perform droplet-based single-cell transcriptomic profiling in a clinical laboratory. Using this instrument we profiled thousands of single cells derived from synovial tissue obtained from 5 RA patients immediately after joint surgery. This methodology allowed us ...
In conclusion, obtained molecular data support the regenerative and immunomodulatory properties of ASCs when interacting with osteoarthritic joint environment. Keywords: osteoarthritis; synovial fluid; ASCs; secretome; extracellular vesicles; miRNAs; cytokines; chondrocytes...
the anti-inflammatory effect ofSteviaextract is clear against MSU-induced gouty arthritis, decreasing the ankle joint edema, which is the first hallmark of the inflammatory process, and, in addition, improving the histological structure of the synovial tissue by modulating inflammatory signaling pathways...