When bound to nucleotide, the phosphates force the P-loop to be in an extended conformation with the side chain of F723 outward (grays, labeled 1 and 3). When bound to a TKI (osimertinib shown, magenta), F723 often folds under the P-loop (black, labeled 2) where the phosphates of ...
reader. Optimization of the probe Z-height was performed using the read height auto-adjust function in the Gen5 software on the Synergy H1 and Neo (Figure 3) resulting in optimal signal sensitivity at heigh...