Cytoskeletal damage that may impede synaptic transmission in the limbic regions during prion disease. a A schematic diagram (Biorender) of a neuron showing the cytoskeletal markers analyzed, including filamentous actin (F-actin), microtubules (beta-tubulin), and neurofilament (NF-L). b–d Immunofluo...
aSchematic diagram of the dorsal hippocampus showing the region at the border between S.P. and S.O. in which gephyrin aggregates were detected.bSchematic representation of the four genotypes analyzed in this study.cPhotomicrographs of the border region between S.P. and S.O. labelled with DAPI...
In this assay, peptides labelled with FITC and biotin at the N- and C-terminus, respectively, were incubated with lysates of homogenates or synaptosomes from control or AD hippocampus. Non-cleaved peptides were removed using streptavidin beads. Levels of FITC groups released by the peptide ...
1j–p). This morphological size reduction was accompanied by a change in the distribution of active zone synaptic vesicle release sites labelled by the marker Bruchpilot (Brp)20 (Fig. 1j-o), which increased in number until middle age before declining in older animals, consistent with prior ...
32 For intramolecular FRET experiments designed to explore if KET would prevent NMDAR-IgG-elicited impairments in CaMKIIα activity, neurons were transfected to express the FRET-based sensor Green-Camuiα in which the N- and C-termini of CaMKIIα are labelled with monomeric enhanced GFP (mEGFP,...
The immunological synapse is a molecular hub that facilitates the delivery of three activation signals, namely antigen, costimulation/corepression and cytokines, from antigen-presenting cells (APC) to T cells. T cells release a fourth class of signaling
The diagram in the top panel shows the overall structure of the complex. The bottom left and right panels show the heterodimerization interface of the RIM2a ZF domain with the Munc13-1 C2A domain and C-terminal a-helix, respectively. The mutated side chains are labelled. (c) ITC analysis...
After confirming germline transmission and Mendelian inheritance of the Cdc42ep4fl and Cdc42ep4 À alleles, the lines have been deposited to RIKEN Bioresource Center (RBRC04894 and RBRC09539, respectively). Balance beam and rota-rod tests. All systematic behavioural tests were con- ducted on ...
However, further studies using the same model could reveal synaptic transmission in response to the action potential of hippocampal neurons. This study type could reveal the functional aspects of and recovery from cognitive impairment in the AD mouse model by using behavioural testing. In conclusion,...
For visually examining the occurrence of the native oxidation event, we performed the microscopic structure observation of the InSe A-FET using high-resolution-transmission electron microscopy (HRTEM). The cross-sectional image and the corresponding energy-dispersive X-ray spectroscopy (EDS) element mapp...