Zhuangzhi Cong等人利用CRISPR/Cas9技术分别在原代结肠上皮细胞、SW480和HCT116中引入了SNP rs6854845 G > T突变，并且检测了这一突变对染色体高级结构和基因表达的影响。结果表明这一突变只影响正常结肠上皮细胞的染色质高级结构和基因表达，而SW480和HCT116结肠癌细胞对此耐受，从而暗示了细胞癌变会逃脱正常多态性对...
Gene expression of apoptosis genes, Bax and Bcl2 , was assessed by qPCR . The viability of SW 480 and HCT 116 cells treated with recombinant L. lactis was significantly reduced. A significant change was observed in the ratio of Bax/Bcl2 expression in HCT 116 cells only following treatment ...
Zetalife公司Advanced DNA/RNA Transfection Reagent转染HCT116、SW480、HT29细胞(极难转染细胞)见刊文章,可替代慢病毒感染,节约包病毒时间。 发布于 2020-06-05 00:05赞同13 条评论 分享喜欢收藏申请转载
Zhuangzhi Cong等人利用CRISPR/Cas9技术分别在原代结肠上皮细胞、SW480和HCT116中引入了SNP rs6854845 G > T突变,并且检测了这一突变对染色体高级结构和基因表达的影响。结果表明这一突变只影响正常结肠上皮细胞的染色质高级结构和基因表达,而SW480和HCT116结肠癌细胞对此耐受,从而暗示了细胞癌变会逃脱正常多态性对染色...
HCT116是1979年M.Brattain等从患结肠癌的男性病人中分离的三株恶性细胞之一。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性,形成上皮样的肿瘤。 人结肠癌细胞,SW480[SW-480] SW480源自一位51岁白人男性患者的原位直肠腺癌,而SW620源自同一病人一年后的淋巴结转移灶。
细胞:SW480, SW620, HCT-8, and HCT-116 colorectal cancer cells and NCM460 normal human colon mucosal epithelial cell lines2022年影响因子/JCR分区:4.501/Q2 MicroRNA-1276 Promotes Colon Cancer Cell Proliferation by Negatively Regulating LACTB. Cancer Manag Res. 2020 Nov 26;12:12185-12195. doi:...
html. colon cancer cells (HCT-116, SW480, HT-29) were seeded at 50% confluency for 24 h in six-well and 96-well plates. Plasmid (10 ug per-well for six-well and 0.5 μg per-well for 96 plates) and the Advanced DNA RNA transfection(zeta life,USA) reagent were mixed in equal ...
HCT116[HCT116]细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细胞生长特性:贴壁或悬浮,详见细胞说明书部分;细胞背景资料:详见相关文献介绍 T24细胞系;细胞传代方法:1:2传代;细胞形态特性:上皮样;细胞生长特性:贴壁生长;细胞背景资料:该细胞源自一位81岁白人女性患者的膀胱移行...
Details about plasmid containing the AURKA gene can be found at colon cancer cells (HCT-116, SW480, HT-29) were seeded at 50% confluency for 24 h in six-well and 96-well plates. Plasmid (10 ug per-well for six-well and 0.5 μg per-well for 96 plates) and the Advanced DNA RNA...
found at https://www.vectorbuilder.cn/vector/VB190802-1063ncc. html. colon cancer cells (HCT-116, SW480, HT-29) were seeded at 50% confluency for 24 h in six-well and 96-well plates. Plasmid (10 ug per-well for six-well and 0.5 μg per-well for 96 plates) and ...