对有关猿猴病毒SV40可能在人类癌症的发生中起 重要作用这一问题却未提及.其理由是SV40已被证明在体外能将正常人细胞转化为癌细胞。[1]SV40病毒的基因组是一种环形双道链的DNA，基因组5.2kb，病毒的直径45nm，病毒成熟部位细胞核，无被膜，62个核壳粒亚单位，这种大小很适于基因操作。同时它内也是第一个完成基因组DN
增强子是真核基因表达调控中可增强启动子活性的顺式作用元件.近年来,人们在 SV40,痘苗病毒,呼吸道合胞病毒,大肠杆菌及人乳头瘤病毒等基因组中发现了可 在原核细胞 中具增强子功能的DNA序列,称为原核增强子样序列C1~Lo].我们发现萤火虫荧 光素酶基因
1.6转基因小鼠的建立采用显微注射的方法将3.8kb的H+-K+ATPase β promoter/SV40T基因片段注射入C57BL/6×CBA F1代小鼠受精卵的雄原核中,并移植假孕母鼠输卵管中使其发育生长。 1.7PCR方法筛选转基因小鼠剪取2周龄小鼠尾尖(1cm左右) ,蛋白酶消化法提取基因组DNA.根据SV40T抗原基因序列设计特异性引物:上游引物为5...
thepVR10122Xwas312foldhigherasthatofcontrolplasmid1ItissuggestedthattherecombinantplasmidpVR10122 Xcanbeexpressedinmammaliancellline,andhastransactivatingeffectonSV40earlypromoter1 KeywordsHBVXgene;eucaryoticexpression;transactivatingeffect;SV40earlypromoter X开放读码框架是HBVDNA中最小的一个开 放读框,编码有145～...
2) SV40 promoter SV40启动子 1. A zinc finger peptide that specific to a 9 bp target on SV40 promoter was got by screening zinc finger phage display libraries. 将通过筛选噬菌体展示锌指文库得到的特异作用于SV40启动子上9bp序列的三锌指结构的序列插入含有TAT蛋白的蛋白质转导结构域的表达载体pET-TA...
This is primarily of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements. The occurrence and magnitude of such effects will depend on several factors: i) the combination and activities of the genetic regulatory elements present on ...
Genomic organization and promoter analysis of the human nicotinic acetylcholine receptor α6 subunit (CHNRA6) gene: Alu and other elements direct transcriptional repression. Gene, 2002, 298(1): 101–108. [4] Andreassi C, Riccio A. To localize or not to localize: mRNA fate is in 3′ UTR ...
1) SV40 SV40 1. Analysis of the Complete Genome Sequence of a Highly DivergentSV40Chinese Isolate; 一株高度变异的中国SV40分离株的全基因组序列分析 2. Role ofSV40in the immortalization of human odontoblast-like cells; SV40在人成牙本质细胞永生化中作用的初步研究 ...
P29817 pGL3-Basic-PDL1-Promoter-mut质粒载体 P29428 pCAGGS DRR mCherry Donor EF1a BFP质粒载体 P29409 pcDNA5-Flag-BRD4-WT质粒载体 pGPU6-ARHGEF28-shRNA2-EnCMV-EGFP-SV40-Neo质粒载体 P29745 pC1-mitoRexYFP质粒载体 P29420 pTK299质粒载体 pLKO.1-U6-CDK1-shRNA3-PGK-Puro质粒载体 ...