For Cell Counting Kit-8 (CCK-8) assay, cells were seeded at a density of 1000 cells/well in 96-well plates with indicated treatment. Then, 10 μl of CCK-8 solution (Targetmol C0005, China) were added to each well and incubated with the cells for 4 h at 37 °C. The absor...
ELISA kit was used to detect the expression of several inflammatory cytokines such as MCP-1 (Cat#432704, Biolegend), IL-17A (Cat#433007, Biolegend), TGF-β (Cat#432507, Biolegend), IL-6 (Cat#1210602, DAKEWE), IL-12p70 (Cat#1211202, DAKEWE) and TNFα (Cat#1217202, DAKEWE) in...
Examples abound. Take the dogmatic exclusion rules of most religions. Can we look again at the Inquisition, or the Tokugawa extirpation of Japanese Christians, or the Holocaust, in new light? One of the Iranian Ayatollahs once said of America — "We don't fear your bombs, we fear your pag...
But for larger cuts a tube of super glue is worth making certain you keep with you in your pocket survival kit. A few drops will seal shut a wound that would have otherwise required sutures. Severe pain: A thousand possibilities exist for the human body to experience pain in survival situa...