Surveyor Nucleaseが、2本鎖の中のミスマッチ箇所を切断する。 電気泳動による検出: サンプル内のDNAを電気泳動で分画し、Surveyor Nuclease処理による2本鎖の切断が起こったかどうかを確認する。複数のバンドが観察されれば、変異型の細胞がテストサンプルに含まれていることが分かる(電気泳...
Heteroduplex cleavage fragment analysis was performed with SURVEYOR Nuclease and the WAVE HS System. SURVEYOR cuts amplicons where there is a heteroplasmic variation. The presence and sizes of these fragments were viewed on the WAVE, see Figure 4. Figure 4. POLG SURVEYOR Analysis. Patient s...
The 14C-urea breath-test for the detection of gastric Campylobacter pylori infection Assessing large-scale surveyor variability in the historic forest data of the original U.S. Public Land Survey Mutation detection using Surveyor nuclease. Surveyor: an infrastructure for internet performance measurements...
T7E1酶切反应体系:Nuclease-Free H2O 7.8 μL;NEB Buffer 2 μL;Hybridization DNA 10 μL;T7E1 nucleases 0.2 μL。Surveyor酶切反应体系:Nuclease-Free H2O 6 μL;0.15 mol/L MgCl2 2 μL;Hybridization DNA 10 μL;Surveyor Enhancers 1 μL;Surveyor nucleases 1 μL。37 ℃的水浴中酶切1 h,2%的...
A simple, high sensitivity mutation screening using Ampligase mediated T7 endonuclease I and Surveyor nuclease with microfluidic capillary electrophoresis. Electrophoresis 33 : 788–796.Huang, M. C., Cheong, W. C., Lim, L. S. & Li, M. H. A simple, high sensitivity mutation screening ...
Results of the surveyor nuclease assay.doi:10.1371/JOURNAL.PONE.0122059.G006Semaan MarwanI. DanielDenner JoachimPLOS ONE
Error correction of microchip synthesized genes using Surveyor nuclease. Nucleic acids research. 2011 doi: 10.1093/nar/gkr887. (In print) [ Cross Ref ]I. Saaem, S. Ma, J. Quan, and J. Tian, "Error correction of microchip synthesized genes using surveyor nuclease," Nucleic Acids Res., ...
Analysis of SCN1A gene for pathogenic mutations in Severe myoclonic epilepsy of infancy (SMEI) by DHPLC, SURVEYOR® Nuclease and DNA sequencing: A sensitive efficient clinical approachSevere myoclonic epilepsy of infancy (SMEI) has been characterized by generalized tonic, clonic and tonic-clonic ...
Surveyor nucleaseIntroduction Infections with Candida glabrata have recently gained worldwide attention owing to its association with long hospitalizations and high mortality rates. This problem is highlighted when the infection is associated with echinocandin resistance, which is used for first-line therapy...
Mutations and polymorphisms in mtDNA detected by "Surveyor Nuclease" in children with manifestations of mitochondrial encephalomyopathies - European Journal of Paediatric Neurologydoi:10.1016/S1090-3798(08)70159-4J. PilchM. AsmanE. JamrozM. Kajor...