The present invention comprises a microtiter plate formed of a substantially rigid, polymeric plate having a substantially flat upper surface and a regular array of similar wells, typically either cylindrical or frusto-conical, each well being defined by a fluid- impervious peripheral wall extending a...
After a 2-h incubation, the supernatant was transferred to a new 96-well plate. The absorbance was measured at 450 nm using a microplate reader (Bio-Rad, USA). All experiments were repeated thrice. Osteogenic differentiation The hBMSCs (1 × 104 cells/mL) were cultured with each group...
The water permeability showed that the water permeability of NPG-N and NPG-F are both higher than NPG-OH due to the higher pore area of NPG-N and NPG-F. And the minimum permeability is more than ~ 4–5 times the values obtained in the prior art, revealing that the NPG membrane may...
By simple extrapolation, we estimate the plateau value to ~114 nmol.m−2, which corresponds to a surface of 1/(NA × 114 nmol.m−2)≈ 14.5 nm2 per Jo molecule; where NA is the Avogadro’s number. This value correlates well with the projected surface area of Jo protein ...
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In the experiment, the L-929 cell suspension was inoculated into a 96 well plate, 100ul per well, with a total of 1 × 104 cells per well, and cultured at 37 °C for 24 h (double cycle) with 5% CO2. After the culture was finished, the culture medium in the culture plate was ...
Based on the average chain length of the polymer molecules as well as the initial amount of probe, it is estimated that roughly 1 % of the polymer molecules contained a fluorescent probe, embedded in the hydrophobic middle block. Figure 1 Overview of the synthesis of PEG 400 -PHA-PEG 400 ...
Wells of a 6-well plate were coated with either 20 μg/ml fibronectin, 25 μM III1-C or 25 μM III 11-C for 1 hr at RT. Wells were washed 3 times with PBS before adding cells. Growing RASMCs were harvested by trypsinization and collected into DMEM + 0.5% BSA + soybean trypsin...
40 μL of cells were then added to wells of a 96-well plate, followed by addition of 200 μL of purified VGJΦ phage stock. For inhibition of retraction with the mshAT70C strain, 25 μL of 50 mM MeOH-PEG-mal was added to the cells prior to the addition of the phage ...
Wilhelmy plate tensiometry, cantilever-based force probes, and atomic force microscopy methods, which we will discuss in later sections). The distinction between contact angles (and surface wetting properties) measured under quasi-static and dynamic conditions is either ignored or not well understood,...