Fig. 2: A time-delay model explains the relative dynamics of surface area and volume synthesis in batch cultures. a A conceptual model for SA/V regulation. The synthesis rates of both surface area and volume scale with current cell volume (Eq. 1, 2), predicting that the time derivative ...
After a 2-h incubation, the supernatant was transferred to a new 96-well plate. The absorbance was measured at 450 nm using a microplate reader (Bio-Rad, USA). All experiments were repeated thrice. Osteogenic differentiation The hBMSCs (1 × 104 cells/mL) were cultured with each group...
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The present invention comprises a microtiter plate formed of a substantially rigid, polymeric plate having a substantially flat upper surface and a regular array of similar wells, typically either cylindrical or frusto-conical, each well being defined by a fluid- impervious peripheral wall extending a...
Considering the drastically reduced area with adhesions, this proves the effectiveness of the PECVD coating with respect to the reduction of adhesion between copper and hot work steel DIN 1.2367. As with the boriding of alloy 718, this reflects a substantial improvement on the adhesion behavior ...
Electrode surface area is a quantity that can be measured in situ via capacitance to understand kinetics of porous electrodes; however, this calculation requires prior knowledge of specific capacitance of a model material with a known, well-defined area, such as glassy carbon. These model materials...
Based on the average chain length of the polymer molecules as well as the initial amount of probe, it is estimated that roughly 1 % of the polymer molecules contained a fluorescent probe, embedded in the hydrophobic middle block. Figure 1 Overview of the synthesis of PEG 400 -PHA-PEG 400 ...
Recently, the importance of surface nanotopography in the determination of stem cell fate and behavior has been revealed. In the current study, we generated polystyrene cell-culture dishes with an omnidirectional nanopore arrayed surface (ONAS) (diameter: 200 nm, depth: 500 nm, center-to-cen...
Understanding the modulations of motor control in the presence of perturbations in task conditions of varying complexity is a key element towards the design of effective perturbation-based balance exercise programs. In this study we investigated the effect of mechanical perturbations, induced by an unsta...
As a post-processing technique of ultrasonic phased array, the total focusing method (TFM) is a delay-and-sum imaging technique based in the time domain where the area of inspection is discretized into a grid and the signals from every transmit–receive pair are subsequently focused at each pi...