although the highest levels are observed in mature B cells.11–13 c-rel knockout mice developed normally with no effects on B-cell maturation but do exhibit some immunological defects, including reduced B-cell proliferation and activation, abnormal germinal centres and reduced number of marginal zon...
qRT-PCR analysis of the patient cohort with Union for International Cancer Control/American Joint Committee on Cancer stage III colorectal cancer (T, n = 76 patient samples) showed a highly significant reduction of SASH1 expression (upper right panel; Mann–Whitney U test; P < .0001) compared...
This resulted in a dramatic reduction in anchorage-independent growth (Figure S2A), a hallmark of cellular transformation. Thus, we next used CRISPR/Cas9-mediated genome editing to ask whether reverting an endogenous LOF allele to WT would also rescue cell growth. We used DLD1 colon cancer ...
aTop: diagram of the PB vector pGG13454. Bottom: diagram of the screening strategy used to identify proteins involved in HTT-dependent toxicity. Created with BioRender.com.bNumber of surviving cells scored by quantification of Crystal Violet (CV) staining-positive colonies upon treatment for 48 ...
Recently, we discovered that oxidative stress and antioxidants control the subcellular distribution of PML. The antioxidant sulforaphane (SFN) is a potent inducer of cytoprotective genes [149]. The precursor of SFN, glucoraphanin, is abundant in cruciferous vegetables with its highest concentration found...
Recently, we discovered that oxidative stress and antioxidants control the subcellular distribution of PML. The antioxidant sulforaphane (SFN) is a potent inducer of cytoprotective genes [149]. The precursor of SFN, glucoraphanin, is abundant in cruciferous vegetables with its highest concentration found...
Resampling was carried out with 500 random permutations of the data. The multiple level of significance was α=0.01. Negative, one-sided significance test was applied. The β-sums statistic of a gene set was calculated only from the three highest β-values. Data clustering and visualization was...
9G). A significative decrease in angiogenesis, determined by a reduction of the area occupied by CD31+ cells in the tumor/peritumor areas, was observed in mice treated with guadecitabine/ICBs compared to guadecitabine or ICBs alone (Fig. 9H). The serum levels of angiogenic chemokines CXCL5 ...
(PT) were obtained using MELT version 2.1.548. To discard possible sequencing artifacts, candidate somatic insertions were further filtered including calls supported with a minimum of three split-reads, with the highest accuracy assessment for breakpoint detection and passing all internal filters (...
Reactome (http://www.reactome.org/) as well as as STRING-DB (http://string-db.org/) web-interfaces were used to perform similar enrichment studies on the top predictions. Results Comparison with other computational approaches for PTEN ceRNA prediction In recent work, multiple databases and ...