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Then, Cap 1 was added to the synthesized RNA by using the Cap 1 Capping System (Novoprotein, Shanghai, China) and Pseudo-UTP (Ψ-UTP, APExBIO, Houston, TX, USA) was fully substituted for UTP. The Poly(A) tails were added using E. coli Poly(A) Polymerase (Novoprotein, Shanghai, ...