与Super Shift EMSA一起使用的抗体应符合以下标准: (1)识别的结构域抗体应位于蛋白质-核酸复合物的表面; (2)蛋白质-核酸复合物上的结构域不应与蛋白质-核酸复合物的相互作用区域重叠; (3)用于蛋白质印迹的抗体不能与超迁移EMSA一起使用,因为抗体只能识别蛋白质的变性prim结构。; (4)通过注射肽制备的抗体很少...
网络超迁移分析;超变动分析 网络释义
GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure: 1. Prepare Whole Cell Extract. Make sure every solution D GEL SUPER-SHIFT ASSAY/ EMSA Materials/Reagents: See Gel-shift assay Procedure: 1. Prepare Whole Cell Extract. Make sure every solution DOES NOT contain DTT...
Electrophoretic mobility shift assay (EMSA) is a powerful technique to resolve nucleic acid-protein complexes formed with transcription factors in nuclear extracts. Herein is described how EMSA and super-shift assays were used to characterize several complexes produced from binding of transcription factors...