N.G. funded by the Engineering and Physical Sciences Research Council (EP/L504889/1). D.O. funded by Marie-Curie grant 334303 and European Research Council grant 337187. Author information Author notes Nils Gustafsson and Siân Culley: These authors contributed equally to this work Authors ...
Beyond sophisticated techniques of point-spread function engineering [16,17], methodological approaches using standard objective lenses and instrumental equipment are often summed up as localization microscopy. These approaches are based on the fundamental concept of optical isolation of molecular labels by ...