Gene fragments (SEC61B, KASH domains of Nesprin-1 and Nesprin-2) were amplified by PCR using A549 cDNA as template. mCherry was amplified from pLVX-mCherry-N1 (YouBio, VT2003) by PCR. Primer sequences were listed in Supplementary Table 4. PCR fragments were purified and cloned into pLV...
Staring into thick bush, full of shadows and tangled plants, I’m always aware there COULD be something in there that I don’t know about. It’s probably something I’ve heard of before. But what if it’s something hidden, something unknown? And once I started thinking about this,Crypto...