In the SUMO system, the ATP-requiring, dimeric SAE1–SAE2 (also known as AOS1–UBA2) enzyme that makes the first high-energy thioester bond with mature SUMO that is necessary for the subsequent transfer steps. SUMO E3 ligase The protein that brings the SUMO-charged E2 enzyme and substrate...
mTOR-regulated senescence and autophagy during reprogramming of somatic cells to pluripotency: a roadmap from energy metabolism to stem cell renewal and aging. Cell Cycle 10, 3658–3677 (2011). 75. Pan, Y. et al. Human germline and pan-cancer variomes and their distinct functional profiles. ...
There are many factors that affect weight loss, but it ultimately comes down to energy balance: "You need to be in a calorie deficit," Young says. "The input side of the equation is the food we eat, and the output side is physical activity." To be in acalorie deficit, you need to...
Peak 0.40 0.33/0.54 2.10 3.75/4.21 Remote 0.58 0.45/0.61 1.25 1.81/3.84 FOMe 25–1.8 Å Acentric 0.655 Centric 0.557 DM modified phases 0.795 Refinement 25–1.6 Å Number of reflections > 1.0σ 47552 Nonhydrogen atoms Protein 2418 Water 432 Rcrystf 0.190 Rfreeg 0.251 Rms...
The problem was Kakizoe had used up a lot of his energy at this point, and when he went for the force at at the ring's edge, Kokkai was able to successfully counter with a nice kote-nage throw. Goliath wins...this time...to move to 5-4 while Kakizoe falls to 1-8. M3 ...
Maximize the amount of impact your posts will make so you don’t waste your time and energy. Here are some tips. Don’t Post at High-Traffic Times Posting at peak times will hurt your organic reach. At first glance, that seems counterintuitive. But it makes sense if you think about it...
The twelve most intense ions with charge states ≥2 were sequentially isolated to a target value of 1 × 105 with a maximum injection time of 45 ms and fragmented by HCD (higher-energy collisional dissociation) in the collision cell (normalized collision energy of 28%) and detected in the ...
If we are not enough as we are, of course we will expand vast amount of energy trying to BECOME enough in the future. But that’s a trap. We cannot become what we already are, and the search obscures this recognition. Does this mean we become passive, lazy, indifferent and don’t ...
To test for interactions, column peak fractions were analysed by SDS-PAGE (Fig. 2a; right panels show peak fractions). In this setting, Crm1 was the only transport receptor that significantly co-eluted with the RanBP2-3/4 complex (Fig. 2a,b). Stable interaction of Crm1 was also ...
MS/MS scans were acquired in HCD mode with a normalized collision energy of 25 and resolution of 30,000 using a TopN = 5 method, with a target AGC of 2E4 and a maximum injection time of 1000 ms. Data processing The SUMO proteome MS data were analyzed using MaxQuant (version ...