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An old method in which high energy particles (e.g. electrons) were used to inactivate lyophilised proteins and give an indication of the molecular weight (proportional to the target size). A macrocyclic antifungal compound, structurally unrelated to geldanamycin, but that competes for the same bind...
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ATP-dependent activation of mature Ub/Ubl by the E1 enzyme results in a high-energy E1-Ub/Ubl thioester linkage. Yeast Smt3 E1 is the Aos1/Uba2 heterodimer (Johnson et al. 1997). The Ub/Ubl-E1 thioester is then transferred to an E2 enzyme. E2 utilizes the high-energy thioester to ...
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A full-mass scan was performed between m/z 350 and 2000, followed by MS/MS scans of the five highest-intensity parent ions at 35% relative collision energy. The AGC of fluoranthene anions was set at 3 × 105 and the reaction time was set at 200 ms during ETD. For the ...
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regulations). It builds on the foundation of NIS1, expanding its scope to cover a broader range of sectors, including digital service providers and online platforms. The directive aims to enhance the overall cybersecurity posture across critical sectors, such as energy, transportation, health, and...
In an ATP-consuming reaction, SUMO is then activated by adenylation, enabling it to form an energy-rich thioester bond with the catalytic cysteine of the heterodimeric E1 enzyme, composed of the Aos1/Uba2 (Sae1/Sae2) subunits (31). Next, SUMO is passed to the catalytic cysteine of the ...
ΔiG is the solvation free energy gain upon formation of the interface. NHB and NSB are the numbers of hydrogen bonds and salt-bridges across the interface. Only interfaces burying more than 100 Å2 of accessible surface area are listed. Supplementary Figure 3 Structural comparison of the SUMO...