High sensitivity, Accurate and reproducible data, Low signal-to-noise ratio, High resolution, Rapid and low-cost, Creative Bioarray is offering high quality SRB assays.
Sulforhodamine B (SRB) cell cytotoxicity assay has been widely used for drug toxicity screening and cell proliferation detection against different types of cancerous and non-cancerous cell lines. In addition, this assay does not depend on the metabolic activity of the cells and therefore shows less...
Results of the SRB assay were linear with cell number and cellular protein measured at cellular densities ranging from 1 to 200% of confluence. Its sensitivity is comparable with that of several fluorescence assays and superior to that of Lowry or Bradford. The signal-to-noise ratio is ...
various malignant cell lines derived from human blood, breast, cervix, colon, pancreas, and lung cancers have been used and the standard assays for estimation of cell viability, that is, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and sulforhodamine B assays and trypan...
Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies...
Various assays are used to evaluate the cytotoxic effect of chemicals on cultured cells. The sulforhodamine B (SRB) colorimetric assay is based on the ability of the SRB dye to bind basic amino acid residues on proteins. In contrast, the MTT (dimethylthiazol-diphenyltetrazolium bromide) colorimetri...
Sulforhodamine B (SRB) is often used as a membrane-impermeable polar tracer or used for cell density determination via determination of cellular proteins (cytotoxicity assay). The SRB assay has been used to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies...
Total organic carbon (TOC) and chemical oxygen demand (CODCr) assays show that the degradation rate of SRB is much higher when irradiated with UV and sunlight compared with visible light. The temporal concentration changes of SRB illustrated a first-order reaction and the rate constant, k, is ...
The sulforhodamine B (SRB) assay was developed by Skehan and colleagues to measure drug-induced cytotoxicity and cell proliferation for large-scale drug-sc... W Voigt - 《Methods in Molecular Medicine》 被引量: 241发表: 2005年 Comparison of 5 microplate colorimetric assays for in vitro cytotoxic...
SRB has better linearity and higher sensitivity than other colorimetric assays, and is comparable to those achieved with standard fluorescent dye staining methods [16,17]. The method is very cheap as it requires only a multiwell reader, has a stable endpoint, and the reagent can be stored ...