The aim of this work was to investigate whether product inhibiton of sucrose-phosphate synthase (SPS) by sucrose-6-phosphate (sucrose6P) plays a significant role in feedback regulation of sucrose synthesis in spinach leaves. The amount of sucrose6P in leaves was very low (0.6-0.7 nmol mg1 ...
In the process of sugar accumulation,the activities of sucrose phosphate synthase and sucrose synthase were higher. 在龙柚糖积累过程中,蔗糖合成酶(SS)和蔗糖磷酸合成酶(SPS)活性较高;而蔗糖中性转化酶(NI)活性则随着蔗糖的积累而降低。 3. The paper deals with research advances of sucrose phosphate syn...
Spinach leaf sucrose phosphate synthase activation by glucose 6-phosphate and inactivation with inorganic phosphate. FEBS Lett. 153, 293-8.Doehlert, D.C. and Huber, S.C., Spinach Leaf Sucrose Phosphate Synthase: Activation by Glucose-6-Phosphate and Interaction with Inorganic Phosphate, FEBS Lett...
Here, we demonstrate that the sucrolytic activity of RcSUS1, the dominant sucrose synthase isozyme expressed in developing castor beans, is allosterically inhibited by T6P. The feedback inhibition of SUS by T6P may contribute to the control of sink strength and sucrolytic flux in heterotrophic ...
蔗糖磷酸合成酶(sucrose phosphate synthase, E.C.2.4.1.14)是一种可溶性酶,最适pH值约为7.0,低丰度(不到可溶性蛋白的0.1%),不稳定,存在于细胞质中。SPS的活性主要受酶蛋白含量、变构效应因子(G-6-P和Pi)和可逆丝氨酸磷酸化调节。Winter和Huber认为SPS的调控是通过与14-3-3蛋白结合并磷酸化和异构化的遗传...
Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells are provided.doi:USRE38446 E1Van Assche, CharlesLando, DanielleBruneau, Jean MichelVoelker, Toni Alois...
It is argued that, although one of the functions of PFK(PP;) is production of pyrophosphate for sucrose breakdown via sucrose synthase, the key role of the enzyme is the maintenance of the cytosolic concentration of pyrophosphate according to the supply and demand of the latter....
sucrose synthasetrehalose‐6‐phosphateIn plants, trehalose 6‐phosphate (T6P) is a key signaling metabolite that functions as both a signal and negative feedback regulator of sucrose levels. The mode of action by which T6P senses and regulates sucrose is not fully understood. Here, we ...
Expression of sucrose: fructan 6-fructosyltransferase (6-SFT) and myo-inositol 1- phosphatesynthase (MIPS) genes in barley (Hordeum vulgare) leaves. J. Plant Physiol., 158: 635-643.Wei J-Z, Chatterton NJ, Larson SR (2001) Expression of sucrose:fructan 6-fructosyltransferase (6-SFT) and ...