The protein with sucrose transporter activity, is a sucrose transporter membrane protein involved in the transmembrane transport of sugar, cane sugar is involved in the process of accumulation of a key protein. 通过基因工程手段对该蔗糖转运蛋白基因的利用,可以有目的提高植物蔗糖利用效率和产量. Through ...
Evidence has been provided that SWEET transporter activity could be modulated by forming dimers with interactions between cytosolic C termini with other SWEETs13. Due to the synergistic action of PpSWEET9a and PpSWEET14 in sucrose efflux from source leaves, we utilized the split-ubiquitin yeast two...
Whether ScrB ofT. thermosaccharolyticumG3-1 was a secretory enzyme was first analyzed by prediction tools, and the results showed no significant signal peptide was predicted inscrBby SignalP-5.0 [35], while transmembrane helices was captured by DeepTMHMM [36]. Four putative signal peptides pred...
Secondly, their proteins contain the 12 transmembrane helices (Figure 2), coined as distinct characteristic features of all members of the GPH cation symporter family to which all the known plant SUTs belong [10, 11], and the consensus sequence derived from the highly conserved region of ...
Von Heijne G (1986) The distribution of positively charged residues in bacterial inner membrane proteins correlates with the transmembrane topology. EMBO J 5:3021–3027 Google Scholar Wagner E, Götz F, Brückner R (1993) Cloning and characterization of thescrA gene encoding the sucrose-specific...
Maltase B1 (GB54549) and two other genes involved in energy meta- bolism, GB50596 (GO term: oxido-reductase activity) and GB48029 (GO term: acyl carnitine transporter activity), were top predictors for the HFCS vs. Sucrose comparison. Consistent with class prediction analyses, Gene Ontology...
More importantly, a 3D structural model of rice sucrose transporter OsSUT1 was built using known crystal structures of transporters from E.coli as templates. Based on the predicted model, six charged amino acids in transmembrane spans were selected for mutagenesis, five of which turned out to be...
The sequences are highly conserved and those carriers display the typical 12 transmembrane 伪-helices of members of the Major Facilitator superfamily. Yeast expression of those carriers indicate that they are all influx carriers, all cotransport sucrose and proton and that their affinity for sucrose ...
It was predicted that the protein exhibited 11 transmembrane spanning domain and the histidine residue (H) was conserved in the extracellular loop. Subcellular localization investigation indicated that RuSUT2 protein was localized in the plasma membrane. The phylogenetic comparisons revealed that RuSUT2 ...
It was predicted that the protein exhibited 11 transmembrane spanning domain and the histidine residue (H) was conserved in the extracellular loop. Subcellular localization investigation indicated that RuSUT2 protein was localized in the plasma membrane. The phylogenetic comparisons revea...