Partially purified sucrose-6-phosphate phosphatase (SPP) from rice leaves showed an absolute requirement for Mg2+, was very specific for sucrose-6-phosphate (sucrose-6-P) and was not significantly inhibited by sucrose up to 100 mM at saturating (2 mM) or cytoplasmic (50 [mu]M) sucrose-P ...
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In a second step, the disaccharide-phosphate is dephosphorylated by sucrose-phosphate phosphatase (SPP, EC 3.1.3.24), a highly specific phosphatase that displaces the reversible SPS reaction from equilibrium in vivo yielding sucrose (Cardini et al., 1955). The two-step pathway can be summarized...
Bioinformatic analysis indicates that sucrose phosphate synthase (SPS) contains a putative C-terminal sucrose phosphate phosphatase (SPP)-like domain that may facilitates the binding of SPP. If an SPS–SPP enzyme complex exists, it may provide sucrose biosynthesis with an additional level of regulation...
We conclude that SPS may possibly interact with SPP, contributing to a more effective sucrose synthesis. 展开 关键词: enzyme activation rice sucrose-phosphate phosphatase sucrose-phosphate synthase sucrose synthesis DOI: 10.1111/j.1365-2184.1996.tb00985.x ...
然后蔗糖磷酸酯酶(SPP) 将蔗糖-6-P迅速降解为蔗糖和磷酸根离子, 而SPS 和SPP 是以复合体的形式存在植物体内, 因此SPS 催化蔗糖生成实际是不可逆的合成过程(Huber and Huber,1996)。 This step response is reversible.Then sucrose phosphatase (SPP) sucrose - 6-P rapid degeneration for sucrose and ...
Sucrose metabolism enzymes:Enzymes catalyzing sucrose synthesis and hydrolysis regulate sucrose content in sugarcane. Sucrose phosphate synthase (SPS) and sucrose phosphate phosphatase (SPP) are responsible for sucrose synthesis. SPS is the key enzyme regulating sucrose synthesis which can be active or...
(SPS, EC 2.4.1.14) and sucrose-phosphate phosphatase (SPP, EC 3.1.3.24) yielding free sucrose and inorganic phosphate (Pi)[8]. Thehydrolysisof the intermediate, sucrose-6P, leads to an essentially irreversible synthesis pathway that provides an efficient production of sucrose, even at low ...
In this study, molecular approaches were conducted to investigate expression profiling of sucrose phosphate synthase (SPS), sucrose phosphatase (SPP) and 14-3-3, which are importance as regulatory points to modulate availability of sucrose in cassava. In source organs, expression of cassava (Manihot...