This study aims to understand tumor heterogeneity and its relation to BC lineage at a single-cell resolution. Chromatin accessibility, TF motif enrichment and their impact on the transcriptome reveal the structure of BC heterogeneity through integration of bulk-RNA/DNA sequencing, scRNA-seq, snRNA-se...
Rhabdomeric photoreceptors of eyes in the terrestrial slug Limax are the typical invertebrate-type but unique in that three visual opsins (Gq-coupled rhodopsin, xenopsin, Opn5A) and one retinochrome, all belonging to different groups, are co-expressed. H
The establishment of cell polarity is a prerequisite for many developmental processes. However, how it is achieved during tip growth in plants remains elusive. Here, we show that the RHO OF PLANTs (ROPs), ROP GUANINE NUCLEOTIDE EXCHANGE FACTORs (RopGEFs), and ROP GTPASE-ACTIVATING PROTEINs (Rop...
We also investigated whether the Opn5B protein is co-expressed with other opsin proteins. Because these anti-opsin antibodies were all raised in rabbits, it was difficult to dually immunostain different opsins in the same sections. Therefore, we exploited the large dimension of the Type-I photore...
After incubation, the cells that successfully traversed the insert were fixed, stained with 1% crystal violet, and subsequently photographed and counted under a visible light microscope (Nikon, Tokyo, Japan). 2.13. Statistical analysis Statistical analyses were conducted using R software (v4.2.0) ...
(version 2.14.0/1.54f), a widely recognized tool for image analysis in biological research. Measurements were conducted by drawing a line across the largest dimension of each organoid, ensuring that the measurement reflected the maximum diameter. This method was uniformly applied to all visible ...
To shed light on the discrepant classification of cells as being myofibroblast-like CAFs or pericytes in tumor samples, we reanalyzed a publicly available head and neck squamous cell carcinoma (HNSCC) dataset19and put it in comparison with our dataset. Puram et al.19classified the tumor fibroblas...
Optical transmission is a widely used technique that uses the interaction of light and viral molecules to identify viruses in a variety of materials. This method involves using incident laser beams characterized by different wavelengths within the visible and infrared spectrum. In this study, we ...
Intrahepatic cholangiocarcinoma (iCCA) is a highly heterogeneous cancer with limited understanding of its classification and tumor microenvironment. Here, by performing single-cell RNA sequencing on 144,878 cells from 14 pairs of iCCA tumors and non-tumo
Disclosed is number coding of pairs (“doublets”) or small sets (“multiplets”) of solid phase carriers which provides distinguishable subtypes of a given type of such carriers, where