WW domain-containing protein 2WW domainBinding affinityComputational modelingAlanine-scanning mutagenesisWW domains harbor substrates containing proline-rich motifs, but the substrate specificity and binding mechanism remain elusive for those WW domains less amenable for structural studies, such as human W...
WW domain-containing protein 2 WW domain Binding affinity Computational modeling Alanine-scanning mutagenesis 1. Introduction WWP2 (WW domain-containing protein 2), is an E3 ubiquitin ligase belonging to the NEDD4-like protein family [1]. The nine members of the family share a similar domain arch...
Similar to the TGD system and disparate from other Gram-negative SBD-containing proteins, the SBD-containing proteins of the Mla and Lpt systems are membrane-anchored by an α-helical transmembrane domain. To our knowledge, the abundance of system components has not yet been verified for either ...
We generated fragments of simian virus 40 large tumor antigen (T antigen) by tryptic digestion and assayed them for helicase activity and helicase substrate (mostly single-stranded DNA)-binding activity in order to map the domain sites on the protein. The N-terminal 130 amino acids were not re...
英文名称: Dnak Substrate Binding Domain C-terminal E.Coli Recombinant 总访问: 245 国产/进口: 进口 半年访问: 17 产地/品牌: prospec 产品类别: 细胞生物学试剂 规格: HSP-009 最后更新: 2024-12-5 货号: CAS 号: 参考报价: 询价 立即询价 电话咨询 ...
The residues in gray represent scaffold residues from the constant part of the light chain’s variable domain. Analysis of the engaged interfaces in all four Fab-RNA structures using PDBePISA (http://www.ebi.ac.uk/pdbe/pisa/) reveals that within the epitope-paratope interface involving Fab ...
Protein disulfide isomerase (PDI) is a multidomain enzyme, operating as an essential folding catalyst, in which the b′ and a′ domains provide substrate binding sites and undergo an open–closed domain rearrangement depending on the redox states of the
3) The deletion of the C-terminal Domain I perturbed the stability of the dimer. 4) Exchanging the central domains perturbed the conformational stability of the protein. Altogether, our results suggest the existence of two similar subdomains in the C-terminal domain of DnaJ that could be ...
3 The abbreviations used are: Hsp70 heat shock protein of 70 kDa JDP J domain-containing protein ER endoplasmic reticulum ERAD ER-associated degradation ss signal sequence HA hemagglutinin TRITC tetramethylrhodamine isothiocyanate. © 2009 ASBMB. Currently published by Elsevier Inc; originally published...
Although inactive by itself in stimulating degradation, the substrate binding domain of E. coli SspB mediates the ssrA-tag binding and dimerization activities of the full-length protein. For example, the purified 1–117 fragment of SspB bound to a fluorescently labeled synthetic peptide containing th...