Luminescent: Produce light upon reaction with an enzyme, offering high sensitivity for detecting low analyte levels. Often used in WB with peroxidase labels. Colorimetric: Cause a visible color change measurable by absorbance, useful for straightforward, quantifiable assays. Often used in ELISA. ...
The substrate is supplied as a stable ready-to-use component solutions. Sufficient material is provided to process approximately 4000 cm2 of membrane or 25-30 mini blots.CatCat
Western BLoT Hyper HRP SubstrateQuantitative detection even for low-abundance proteins. Strong signal when high dilution factor for the primary antibody is used.FemtogramCCD camera, X-ray filmNitrocellulose, PVDF Western BLoT Ultra Sensitive HRP SubstrateHighest sensitivity in the...
DAB 即二氨基联苯胺(3,3’-diaminobenzidine),辣根过氧化物酶(Peroxidase)最常用的生色底物,在过氧化氢的存在下失去电子而呈现出颜色变化和积累,形成浅棕色不溶性产物,在加金属镍盐的情况下,不仅增强了显色强度,而且使显色呈鲜艳的紫蓝色,适用于免疫组化及各种印迹显色法。
显色步骤1、执行常规 SDS-PAGE、转膜和 Western Blot 封闭、一抗孵育、二抗孵育操作。2、二抗适用 HRP 标记二抗,适用恰当的稀释比例。3、制备 ECL 工作液:A 液和 B 液等体积的混合,例如 1ml A 液和 1ml B 液混合,混匀后即可使用。用量以充分覆盖印迹膜为基准,每 10 cm2膜需要大约 1 ml 工作液。4、...
化学发光底物Western BLoT Quant HRP Substrate 收藏产品 加入购物车 ■ 制品内容 (100 ml, for 1,000 cm2of membrane) Code No.: T7102A ■ 制品说明 本制品是辣根过氧化物酶(HRP)的化学发光底物,特别适用于CCD成像系统的Western印迹。使用本制品产生的信号强度与蛋白浓度在较宽广的范围内呈线性关系,能很好地...
To determine the appropriate concentrations, perform a systematic dot blot analysis. ◆Tanon High-sig ECL Western Blotting Substrate requires more dilute antibody concentrations than those used with precipitating colorimetric HRP substrates. To optimize antibody concentrations, perform a systematic dot blot ...
Expose blot to film immediately after treatment with substrate working solution. High background Excessive HRP conjugate used. Dilute conjugate. Inadequate blocking and washing conditions. Verify that the correct blocking buffer, incubation time and number of washes were used. Increase the volume, ...
The secondary antibody should be used as recommend by the manufacturer and optimized as needed. Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.其他推荐产品 Pierce™ 无蛋白 (PBS) 封闭缓冲液 Catalog number: 37572 3,...
Mouse IgG (Part No. 31430). CL-XPosure Film (Part No. 34090) and Pierce ECL Plus Substrate were used for detection. Exposure time was 5 minutes. Hsp86 was detected in HeLa cell lysates according to the following protocol. The lysates were diluted in electrophoresis sample buffer and heated...