The reaction rate of an enzyme can be affected by many factors, and the purpose of this experiment was to find out how an increasing substrate concentration influences the rate of an enzyme activity; we obtained data from recording the absorbance of the samples which contain the same amount of...
Increasing substrate concentration during enzymatic protein hydrolysis results in a decrease in hydrolysis rate. To test if changes in the mechanism of hydrolysis also occur, the enzyme selectivity was determined. The selectivity is defined quantitatively as the relative rate of hydrolysis of each cleavag...
Effect of Temperature, pH, Substrate Concentration and type of Enzyme on the Enzymatic Hydrolysis of Viscera of Red Tilapia (Oreochromis spp.) Protein hydrolysates were prepared from viscera of red tilapia (Oreochromis spp.), an important fish species of great production in Colombia. Hydrolysis wi....
However, thehigh substrate concentrationcan generally lead tosubstrate inhibition, which in turn results in the reaction rate of hydrolysis being decreased and the hydrolysis time being extended, and the extent of substrate inhibition depends on the ratio of the reaction enzyme to substrate [41,42]...
The influence of substrate concentration and hydraulic retention time(HRT) on the hydrogen production by anaerobic microflora was investigated by conducting three series of continuous experiments the individual influences of substrate concentration and HRT. In series I, substrate concentration was increased ...
Fig. 1: lycopene cyclase shows the substrate inhibition effect. aLycopene inhibits its downstream enzyme, lycopene cyclase, through substrate inhibition. Consequently, a higher lycopene formation rate than its subsequent conversion rate into β-carotene could aggravate the imbalance, leading to the build...
shifts between subunits A and B is the largest in TM3 (Supplementary Fig.4). This is consistent with the proposal that the hinge and the difference in local conformation of this helix21between the two subunits control the asymmetric structure of the EmrE dimer and the rate of alternating ...
electrostatic environment is the same in the substrate binding pocket of the human enzyme. In our room-temperature structure of hSHMT2, the substrate binding pocket is occupied by a chloride anion, Cl−. The presence of Cl−can be physiologically relevant as intracellular Cl−concentration ...
The observed difference in rate (1.5 × 10 11-fold) is the largest effect of a simple substituent that appears to have been reported for an enzyme reaction. 展开 关键词: OMP OMP decarboxylase ODCase Orotidine Connectivity effect Effective molarity Transition state Binding discrimination ...
The reported yields are percentages based on intact tRNA analysis. The black box indicates that no reaction product was detected. Initially no acyl-tRNA was detected when MaFRS1 was incubated with 13, but when the enzyme concentration was increased fivefold, acyl-tRNA was detected with mono- ...