Using this modules, a reaction is carried out between an enzyme and substrate. Said cellulose ester-based polymer is e.g. cellulose diacetate, cellulose triacetate. Said enzyme is e.g. amylase, aminoamylase, lipase, esterase, alcohol dehydrogenase, glycerol dehydrogenase.KAJIWARA MASAHIRO...
(Figs.3and4). In this arrangement, two RINGs form a dimer and act as an enzyme on a third RING domain, which acts as the substrate in this reaction. We observe that while rigidity is required to position all the important catalytic residues in the E2 active site optimally (Fig.2and ...
Mass spectrometry is a rapid, sensitive, and accurate quantitative approach for the direct monitoring of enzyme-catalyzed reactions that does not require a... B Bothner,R Chavez,J Wei,... - 《Journal of Biological Chemistry》 被引量: 170发表: 2000年 Increase of reaction rate and sensitivity ...
HRP can be measured with a detection limit of 6.0×10 10 g/L and a linear range of 1.0×10 -9 -4.0×10 -6 g/L. The pure product of H 2 O 2 oxidizing OAP catalyzed by HRP was prepared with chemical method. The enzyme-catalyzed reaction has been investigated with electroanalytical ...
Kinetics of Enzyme Action: Essential Principles for Drug Hunters Few scientists have the knowledge to perform the studies that are necessary to discover and characterize enzyme inhibitors, despite the vested interest the... RL Stein - John Wiley...
An enzyme-immobilized electrode is provided and includes an electrode composed of porous carbon or the like, a phospholipid layer on the electrode (), and enzymes immobilized onto the phospholipid layer. The enzymes are, for example, diaphorase and glucose dehydrogenase. An intermediate layer composed...
This reaction occurs in the bulk phase, so catalysis by enzyme absorbed at the solid-liquid interface plays no role. The value of the parameters in the model (i.e., the mass transfer coefficient of substrate dissolution ( k L), the substrate solubility, and the rate constant for the ...
On the basis of the equations derived previously [Tsou, C. L. (1965) Sheng Wu Hua Hsueh Yu Sheng Wu Wu Li Hsueh Pao 5, 398-408, 409-417] for the substrate reaction during the course of enzyme modification, the kinetic behavior of the system chymotrypsin-substrate-modifier has been studi...
it Is shown that when the membrane has the appropriate permeability to both substrates and the enzyme reaction Is diffusion-limited, the difference in the enzyme electrode current from that of an oxygen-sensitive reference electrode Is proportional to the bulk glucose concentration, Irrespective of ...
Core-shell structured mesoporous silica as acid-base bifunctional catalyst with designated diffusion path for cascade reaction sequences. Chem. Commun. 48, 10541–10543 (2012). CAS Google Scholar Zhang, Y. H. Substrate channeling and enzyme complexes for biotechnological applications. Biotechnol. Adv...